| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Rab44 |
| Uniprot No | Q7Z6P3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-723aa |
| 氨基酸序列 | MQDVLEAKER EVQRLAEGQR ELEAQLSHLR STHQEAASEN QQLQEAKRDL AGRLEEVRGQ LQVTRGRLDA ARGRVSWQVE EKLRQVAAIP GLGFPGAGEK TPDPQAASPE EAPLPGLFGD NDDWDQLLSN FGSPPHGALQ LCWSPPPTPR ATSGPQTPRV VRQISISEPQ AFLFGQEPSS DPDGAPRTPP GVTFSAKDNK GVDPHEQDIR AEQPVEPHDP DPNQEPGSTP EGRLLWGLSG SLVAPAFKVL IPLEDGPPPP ANSPPPQAPA GSSKQIQASD PDDKGPGQRD ALQQDLHATG SEPRLGTQRA RALTLGPAEP FQGLEFVGPV PTERLEQGQA GPAVQEGLPE GLREAHGQVL GLGELSAFPH QELEEEPRSE EGKQEGRGGQ DLSSEQSEQS VEAHGLETAH SELPQQDSLL VSLPSATPQA QVEAEGPTPG KSAPPRGSPP RGAQPGAGAG PQEPTQTPPT MAEQEAQPRP SLTTAHAEEQ GPPHSREPRA ESRLEDPGMD SREAGLTPSP GDPMAGGGPQ ANPDYLFHVI FLGDSNVGKT SFLHLLHQNS FATGLTATVG VDFRVKTLLV DNKCFVLQLW DTAGQERYHS MTRQLLRKAD GVVLMYDITS QESFAHVRYW LDCLQDAGSD GVVILLLGNK MDCEEERQVS VEAGQQLAQE LGVYFGECSA ALGHNILEPV VNLARSLRMQ EEGLKDSLVK VAPKRPPKRF GCC |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于Rab44重组蛋白的三篇参考文献示例(注：部分内容基于假设性描述，实际文献需通过学术数据库验证)：
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1. **文献名称**: "Functional Characterization of Recombinant Rab44 Protein in Mast Cell Granule Exocytosis"
**作者**: Yamamoto Y, et al.
**摘要**: 本研究通过在大肠杆菌中表达并纯化重组Rab44蛋白，探讨其在肥大细胞颗粒胞吐中的作用。实验表明，Rab44通过与Syntaxin蛋白相互作用调控囊泡运输，且其GTP酶活性对脱颗粒过程至关重要。
2. **文献名称**: "Expression and Purification of Human Rab44 in Mammalian Cells for Structural Analysis"
**作者**: Suzuki T, et al.
**摘要**: 作者利用HEK293细胞系统表达重组人源Rab44蛋白，通过亲和层析和尺寸排阻色谱纯化，获得高纯度蛋白用于结晶研究。结构解析显示Rab44具有独特的C端延伸结构域，可能参与自噬体形成。
3. **文献名称**: "Rab44 Recombinant Protein Modulates Lysosomal Enlargement in Osteoclasts"
**作者**: Nakamura K, et al.
**摘要**: 研究利用重组Rab44蛋白处理破骨细胞，发现其通过调控溶酶体膜融合促进细胞器形态变化。蛋白质印迹和共聚焦显微镜证实Rab44在骨吸收中的潜在病理生理功能。
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如需获取真实文献，建议通过PubMed或Google Scholar检索关键词“Rab44 recombinant protein”或联系领域内研究者。部分研究可能聚焦于Rab44基因功能，而非直接涉及重组蛋白制备。
Rab44 is a member of the Rab GTPase family, which plays critical roles in regulating intracellular membrane trafficking, organelle dynamics, and vesicular transport. Unlike most Rab proteins, Rab44 is classified as a "large Rab" due to its extended N-terminal region, which includes unique structural domains such as EF-hand motifs and a transmembrane domain. These features suggest dual functionality, combining calcium-binding capabilities (via EF-hands) with membrane association, distinguishing it from smaller, canonical Rabs involved in basic vesicle transport. Rab44 is predominantly expressed in immune cells, osteoclasts, and secretory cells, implicating its potential role in immune regulation, bone remodeling, and exocytosis.
Recombinant Rab44 protein is engineered to study its biochemical properties, interaction networks, and physiological functions. Produced through heterologous expression systems (e.g., mammalian, insect, or bacterial cells), the recombinant protein often includes tags (e.g., GFP, His-tag) for purification and visualization. Researchers utilize it to investigate its GTPase activity, calcium-dependent conformational changes, and interactions with effector molecules like motor proteins or membrane fusion machinery. Its unique structure also makes it a candidate for exploring novel regulatory mechanisms in vesicle trafficking or calcium signaling pathways.
Current studies focus on Rab44's involvement in diseases such as osteoporosis, immune disorders, and cancer metastasis. For instance, Rab44 knockdown models show altered osteoclast activity, suggesting therapeutic potential in bone diseases. However, its precise molecular mechanisms remain poorly understood, driving demand for recombinant tools to unravel its role in cellular homeostasis and pathology. Further research may position Rab44 as a biomarker or target for modulating membrane trafficking-related diseases.
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