| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | H2BC12 |
| Uniprot No | O60814 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-126aa |
| 氨基酸序列 | PEPAKSAPAPKKGSKKAVTKAQKKDGKKRKRSRKESYSVYVYKVLKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSAK |
| 预测分子量 | 21.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于H2BC12重组蛋白的3篇示例性参考文献(注：以下内容为模拟概括，非真实文献)：
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1. **文献名称**: *"Recombinant H2BC12 protein expression and its role in chromatin remodeling"*
**作者**: Zhang L, et al.
**摘要**: 本研究通过大肠杆菌系统成功表达并纯化了重组H2BC12蛋白，验证了其与DNA的结合能力，并发现H2BC12在体外染色质组装过程中具有促进核小体稳定的功能，可能影响基因转录调控。
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2. **文献名称**: *"Structural characterization of human histone H2BC12 using recombinant protein technology"*
**作者**: Smith JR, Thompson M.
**摘要**: 利用X射线晶体学解析了重组H2BC12蛋白的三维结构，揭示了其核心组蛋白结构域中独特的赖氨酸修饰位点，为研究表观遗传修饰机制提供了结构基础。
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3. **文献名称**: *"H2BC12 recombinant protein as a biomarker for cancer progression"*
**作者**: Chen X, et al.
**摘要**: 通过哺乳动物细胞表达系统制备重组H2BC12蛋白，发现其在多种癌症组织中异常高表达，并可能通过调控细胞周期相关基因参与肿瘤转移。
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**说明**：以上为模拟文献，实际研究中建议通过PubMed、Web of Science等数据库检索真实文献(可结合关键词：H2BC12、recombinant protein、histone variant等)。
H2BC12. a member of the histone H2B family, is a core component of nucleosomes, the fundamental repeating units of chromatin. Histones play critical roles in DNA packaging, gene regulation, and epigenetic modifications. The H2B family consists of multiple subtypes, with H2BC12 (histone H2B type C12) encoded by the *H2BC12* gene in humans. This histone variant contributes to nucleosome assembly, chromatin stability, and dynamic regulation of DNA accessibility during transcription, replication, and repair processes. Dysregulation of H2B subtypes has been implicated in diseases, including cancer and developmental disorders.
Recombinant H2BC12 protein is engineered using molecular cloning techniques, typically expressed in *E. coli* or eukaryotic systems to ensure proper folding and post-translational modifications. It is purified via affinity tags (e.g., His-tag) and validated for structural integrity and functional activity. Recombinant versions enable precise studies of H2BC12's interactions with DNA, other histones (e.g., H2A, H3. H4), and non-histone proteins involved in chromatin remodeling. Researchers utilize it to investigate epigenetic mechanisms, such as histone modifications (acetylation, methylation) and their roles in gene silencing or activation. It also serves as a tool for in vitro nucleosome reconstitution, chromatin-binding assays, and screening for therapeutic compounds targeting chromatin-related pathways. Its applications extend to understanding cellular responses to DNA damage, stem cell differentiation, and cancer biology, where aberrant histone function may drive disease progression. By providing a standardized, contaminant-free protein source, recombinant H2BC12 supports reproducible research in epigenetics and molecular biology.
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