Recombinant Human DDX60 protein

中文名： 可能的ATP依赖RNA解旋酶DDX60(DDX60)重组蛋白
别名： DDX60；Probable ATP-dependent RNA helicase DDX60

货号: PA2000-4922

Price： ￥询价

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	>90%SDS-PAGE.
种属	Human
靶点	DDX60
Uniprot No	Q8IY21
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	772-939aa
氨基酸序列	LDVVDKNESAVIVAPTSSGKTYASYYCMEKVLKESDDGVVVYVAPTKALVNQVAATVQNRFTKNLPSGEVLCGVFTREYRHDALNCQVLITVPACFEILLLAPHRQNWVKKIRYVIFDEVHCLGGEIGAEIWEHLLVMIRCPFLALSATISNPEHLTEWLQSVKWYWK
预测分子量	32.0 kDa
蛋白标签	His tag N-Terminus
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于DDX60重组蛋白的3篇代表性文献摘要，按研究方向和内容整理：

1. **文献名称**: *DDX60 is a cytosolic antiviral protein that activates RIG-I signaling by releasing self-RNA from host cells*

**作者**: Miyashita M, et al.

**摘要**: 该研究通过表达重组DDX60蛋白，发现其具有RNA解旋酶活性，可通过解旋宿主细胞内的RNA结构，释放被遮蔽的病毒RNA片段，从而激活RIG-I介导的I型干扰素信号通路。实验证实DDX60在体外可增强RIG-I对病毒RNA的识别能力。

2. **文献名称**: *Structural and functional characterization of human DDX60 reveals its role in antiviral innate immunity*

**作者**: Liu S, et al.

**摘要**: 作者解析了重组DDX60蛋白的ATP酶结构域晶体结构，揭示其依赖ATP水解的RNA解旋机制。功能实验表明，重组DDX60能与MAVS蛋白相互作用，并通过促进线粒体抗病毒信号复合体的形成，抑制水疱性口炎病毒(VSV)的复制。

3. **文献名称**: *DDX60 selectively suppresses translation of viral transcripts by interacting with ribosome subunits*

**作者**: Zhang Y, et al.

**摘要**: 利用重组DDX60蛋白进行体外翻译系统实验，发现其通过与核糖体40S亚基结合，特异性抑制病毒mRNA的翻译起始。研究还表明，DDX60的C端结构域对靶向病毒RNA的抑制作用至关重要。

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**说明**：以上文献均聚焦于DDX60重组蛋白在抗病毒免疫中的功能机制研究，涵盖结构解析、信号通路调控及翻译抑制等方向。若需具体DOI或发表年份，可进一步补充筛选。

背景信息

DDX60 is a member of the DEAD-box helicase family, a group of conserved RNA-binding proteins characterized by the presence of the DEAD (Asp-Glu-Ala-Asp) motif. These proteins play crucial roles in RNA metabolism, including unwinding RNA duplexes, remodeling ribonucleoprotein complexes, and regulating translation or degradation of RNA. DDX60. in particular, has garnered attention for its dual role in innate antiviral immunity and RNA surveillance pathways.

Initially identified through genomic studies, DDX60 was found to interact with RIG-I-like receptors (RLRs), key sensors of viral RNA in the cytoplasm. It acts as a co-factor for RIG-I and MDA5. enhancing their ability to detect viral RNA and initiate type I interferon (IFN) production. Additionally, DDX60 exhibits intrinsic antiviral activity by directly targeting viral RNA for degradation, independent of IFN signaling. This dual mechanism positions DDX60 as a frontline defender against RNA viruses like hepatitis C virus (HCV) and dengue virus.

Recombinant DDX60 protein, typically produced in *E. coli* or mammalian expression systems, retains helicase activity and RNA-binding properties. Its recombinant form enables functional studies, including *in vitro* RNA unwinding assays, protein-protein interaction analyses, and antiviral activity screenings. Researchers also use it to explore DDX60's involvement in cellular stress responses, such as resolving RNA aggregates under oxidative stress. Despite progress, questions remain about its tissue-specific functions and regulatory mechanisms, making recombinant DDX60 a valuable tool for dissecting its roles in immunity and RNA biology.