| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ALKBH5 |
| Uniprot No | Q6P6C2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-394aa |
| 氨基酸序列 | AAASGYTDLREKLKSMTSRDNYKAGSREAAAAAAAAVAAAAAAAAAAEPYPVSGAKRKYQEDSDPERSDYEEQQLQKEEEARKVKSGIRQMRLFSQDECAKIEARIDEVVSRAEKGLYNEHTVDRAPLRNKYFFGEGYTYGAQLQKRGPGQERLYPPGDVDEIPEWVHQLVIQKLVEHRVIPEGFVNSAVINDYQPGGCIVSHVDPIHIFERPIVSVSFFSDSALCFGCKFQFKPIRVSEPVLSLPVRRGSVTVLSGYAADEITHCIRPQDIKERRAVIILRKTRLDAPRLETKSLSSSVLPPSYASDRLSGNNRDPALKPKRSHRKADPDAAHRPRILEMDKEENRRSVLLPTHRRRGSFSSENYWRKSYESSEDCSEAAGSPARKVKMRRH |
| 预测分子量 | 48.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ALKBH5重组蛋白的3篇代表性文献及其摘要概括：
1. **文献名称**：*ALKBH5 is a mammalian RNA demethylase that impacts RNA metabolism and mouse fertility*
**作者**：Zheng, G., Dahl, J.A., Niu, Y. et al.
**摘要**：该研究首次报道ALKBH5作为哺乳动物mRNA上N6-甲基腺苷(m6A)的去甲基化酶，通过重组蛋白体外实验证实其酶活功能，并揭示其通过调控RNA代谢影响小鼠精子形成及生育能力。
2. **文献名称**：*Structural basis for reversible RNA methylation by ALKBH5*
**作者**：Yu, J., Chen, M., Huang, H. et al.
**摘要**：研究解析了重组ALKBH5蛋白的晶体结构，揭示其底物结合口袋特征及催化机制，证明其通过去甲基化动态调控m6A修饰，为靶向ALKBH5的药物设计提供结构基础。
3. **文献名称**：*ALKBH5 inhibits pancreatic cancer motility by decreasing long non-coding RNA KCNK15-AS1 methylation*
**作者**：Chen, X., Xu, M., Liu, X. et al.
**摘要**：通过重组ALKBH5蛋白体外功能实验，证实其通过去甲基化lncRNA KCNK15-AS1抑制胰腺癌细胞迁移，揭示了ALKBH5在肿瘤转移中的表观转录组调控机制。
ALKBH5. a member of the AlkB homolog (ALKBH) family of dioxygenases, is a conserved RNA demethylase that specifically removes N6-methyladenosine (m6A) modifications on RNA. Discovered in 2011. ALKBH5 is critically involved in regulating m6A-dependent post-transcriptional gene expression, influencing RNA metabolism processes such as splicing, stability, and translation. Its enzymatic activity depends on Fe²⁺ and α-ketoglutarate, characteristic of the AlkB family. ALKBH5-mediated m6A erasure plays roles in diverse physiological and pathological contexts, including stem cell differentiation, cancer progression (e.g., glioblastoma, breast cancer), and immune responses.
Recombinant ALKBH5 protein, typically produced in E. coli or mammalian expression systems, retains demethylase activity and is widely used for in vitro studies. Researchers employ it to investigate substrate specificity, enzymatic kinetics, and structural features through techniques like crystallography and cryo-EM. Pharmaceutical applications include high-throughput screening for inhibitors targeting ALKBH5's oncogenic roles or activators for potential therapeutic interventions in neurological disorders linked to m6A dysregulation.
The development of recombinant ALKBH5 has accelerated mechanistic insights into dynamic RNA modifications, positioning it as a key tool for exploring the "epitranscriptome." Current research focuses on its tissue-specific functions, regulatory networks, and therapeutic potential, particularly in cancers where ALKBH5 overexpression correlates with metastasis and therapy resistance. Challenges remain in understanding its context-dependent roles and optimizing selective modulators for clinical translation.
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