| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | splB |
| Uniprot No | A7X3Q8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 37-240aa |
| 氨基酸序列 | ENNVTKIQDTNIFPYTGVVAFKSATGFVVGKNTILTNKHVSKNYKVGDRITAHPNSDKGNGGIYSIKKIINYPGKEDVSVIQVEERAIERGPKGFNFNDNVTPFKYAAGAKAGERIKVIGYPHPYKNKYVLYESTGPVMSVEGSSIVYSAHTESGNSGSPVLNSNNELVGIHFASDVKNDDNRNAYGVYFTPEIKKFIAENIDK |
| 预测分子量 | 29.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于splB重组蛋白的虚构参考文献示例(内容基于学术文献常见结构模拟，非真实发表论文)：
1. **文献名称**: "Functional characterization of the Staphylococcus aureus splB protease in biofilm disruption"
**作者**: Müller, J., et al.
**摘要**: 本研究通过重组表达并纯化SplB蛋白，发现其丝氨酸蛋白酶活性可降解宿主细胞外基质蛋白(如纤维连接蛋白)，并显著破坏金黄色葡萄球菌生物膜结构，提示其在细菌侵袭和持续性感染中的作用。
2. **文献名称**: "Crystal structure and substrate specificity of SplB, a virulence factor in Staphylococcal skin infections"
**作者**: Tanaka, K., & Novak, R.
**摘要**: 通过X射线晶体学解析SplB重组蛋白的三维结构，揭示其催化三联体特征及底物结合口袋的独特性，实验证明其对表皮角蛋白的特异性水解能力可能与皮肤屏障破坏相关。
3. **文献名称**: "SplB recombinant protein as a potential therapeutic target in murine sepsis models"
**作者**: Chen, L., et al.
**摘要**: 利用splB重组蛋白免疫小鼠后，观察到体内中和抗体可降低细菌载量及炎症因子水平，证明靶向SplB的免疫策略在金黄色葡萄球菌败血症治疗中的潜在价值。
4. **文献名称**: "Expression optimization and enzymatic activity modulation of SplB in Escherichia coli"
**作者**: Gupta, S., & Lee, T.H.
**摘要**: 报道splB基因在大肠杆菌中的可溶性表达条件优化，通过定点突变发现His86残基对其蛋白酶活性的关键作用，为后续抑制剂开发提供基础。
(注：以上文献为模拟生成，实际研究需查阅真实数据库如PubMed。)
**Background of splB Recombinant Protein**
The **splB** gene encodes a critical virulence factor in *Salmonella enterica*, a pathogenic bacterium responsible for gastroenteritis and systemic infections in humans and animals. SplB is part of the *Salmonella* pathogenicity island 2 (SPI-2)-encoded type III secretion system (T3SS), a specialized molecular apparatus that injects effector proteins into host cells. This system is essential for intracellular survival and replication of *Salmonella* within membrane-bound vacuoles, enabling evasion of host immune defenses.
SplB functions as a cysteine protease, playing a key role in processing and stabilizing components of the T3SS apparatus. It cleaves the translocon component SseB, ensuring proper assembly and function of the secretion system. This post-translational modification is vital for the delivery of effector proteins that manipulate host cell processes, such as cytoskeletal rearrangement and immune signaling.
The recombinant splB protein is produced using heterologous expression systems (e.g., *E. coli*), often fused with tags (e.g., His-tag) for purification. Its study provides insights into SPI-2-mediated pathogenesis, host-pathogen interactions, and potential therapeutic targets. Researchers utilize splB recombinant protein to dissect protease-substrate relationships, screen inhibitors, or develop diagnostic tools. Understanding splB's structure and function contributes to broader efforts in combating antibiotic-resistant *Salmonella* strains and designing novel antimicrobial strategies.
In summary, splB is a multifunctional protease central to *Salmonella* virulence, and its recombinant form serves as a valuable tool for microbiological and translational research.
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