| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Krtdap |
| Uniprot No | P60985 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-99aa |
| 氨基酸序列 | MKIPVLPAVVLLSLLVLHSAQGATLGGPEEESTIENYASRPEAFNTPFLNIDKLRSAFKADEFLNWHALFESIKRKLPFLNWDAFPKLKGLRSATPDAQ |
| 预测分子量 | 11.1kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于KRTDAP重组蛋白的示例参考文献(注：以下文献为虚构示例，实际文献需通过学术数据库检索)：
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1. **文献名称**：*Cloning and Prokaryotic Expression of Recombinant KRTDAP Protein for Structural Studies*
**作者**：Smith, J.R., et al.
**摘要**：本研究成功克隆了人类KRTDAP基因，并利用大肠杆菌表达系统实现了重组蛋白的高效表达。通过亲和层析纯化获得高纯度蛋白，经圆二色谱分析证实其二级结构稳定性，为后续功能研究奠定基础。
2. **文献名称**：*Functional Characterization of Recombinant KRTDAP in Epidermal Barrier Formation*
**作者**：Lee, H., & Zhang, Q.
**摘要**：通过体外角质形成细胞模型，发现重组KRTDAP蛋白显著增强细胞间连接蛋白(如claudin-1)的表达，并促进脂质代谢相关基因激活，提示其在皮肤屏障修复中的潜在作用。
3. **文献名称**：*KRTDAP Recombinant Protein Attenuates Psoriasis-like Inflammation in Murine Models*
**作者**：Garcia, M.P., et al.
**摘要**：在小鼠银屑病模型中，腹腔注射重组KRTDAP蛋白可抑制IL-17/IL-23炎症通路，减少表皮增厚和炎性细胞浸润，表明其可能作为新型免疫调节剂用于炎症性皮肤病治疗。
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**建议**：实际研究中，可通过PubMed、Web of Science等平台，以“KRTDAP recombinant protein”、“keratin-associated protein expression”等关键词检索最新文献。若KRTDAP为特定领域术语，需结合具体研究背景细化检索策略。
Krtdap recombinant protein is a bioengineered molecule derived from keratin-associated proteins (KAPs), a family of structural components critical to the formation and integrity of hair, nails, and epidermal tissues. These proteins are rich in cysteine residues, enabling disulfide bond formation that contributes to the mechanical strength and resilience of keratinized tissues. The term "Krtdap" likely refers to a specific KAP variant or a designed hybrid protein optimized for enhanced stability or functional properties.
Recombinant Krtdap is typically produced using expression systems such as *E. coli* or mammalian cell cultures, followed by purification processes to ensure homogeneity. Its design often incorporates tags (e.g., His-tag) to facilitate isolation and downstream applications. Researchers leverage this protein to study keratin-mediated cellular processes, including tissue repair, barrier function, and molecular interactions in dermatological contexts.
Interest in Krtdap stems from its potential biomedical applications. It serves as a model for developing biomaterials for wound healing, drug delivery systems, or biocompatible coatings. Additionally, it aids in investigating genetic disorders affecting keratin structures, such as monilethrix or epidermolysis bullosa. Recent studies also explore its role in modulating inflammatory responses or as a scaffold for tissue engineering.
The development of Krtdap recombinant protein reflects advancements in protein engineering and dermatological research, bridging structural biology with therapeutic innovation. Ongoing optimization focuses on improving yield, bioactivity, and compatibility with human physiology, positioning it as a versatile tool for both basic science and translational medicine.
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