| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | cheA |
| Uniprot No | P07363 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-654aa |
| 氨基酸序列 | MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIFRAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQLDAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQSPRRIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEADQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSNESTSIRVAVEKVDQLINLVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFSRYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKRLAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEVAMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQKMGGHVEIQSKQGTGTTIRILLPLTLAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWKVFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAATILGDGSVALIVDVSALQAINREQRMANTAA |
| 预测分子量 | 78.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CheA重组蛋白的3篇示例参考文献(内容为虚构示例，仅供参考)：
1. **文献名称**：*Expression and functional characterization of recombinant CheA in Escherichia coli*
**作者**：Smith J, et al.
**摘要**：研究报道了通过大肠杆菌重组表达系统高效制备CheA蛋白，并验证其自磷酸化活性及与CheW的相互作用，证实重组蛋白在趋化信号传导中的功能完整性。
2. **文献名称**：*Structural insights into CheA signaling complex by cryo-EM*
**作者**：Lee H, et al.
**摘要**：利用冷冻电镜解析了CheA与CheW、受体蛋白形成的复合体结构，揭示了其构象变化如何调控下游CheY磷酸化，阐明了信号传递的分子机制。
3. **文献名称**：*Kinetic analysis of ATPase activity in engineered CheA mutants*
**作者**：Zhang R, et al.
**摘要**：通过定点突变研究CheA激酶结构域的ATP结合位点，发现关键残基对ATP水解速率的调控作用，为设计趋化通路工程化改造提供依据。
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**注**：以上文献为示例，实际引用时请查询真实数据库(如PubMed、Web of Science)获取最新研究。
CheA is a central histidine kinase in the bacterial chemotaxis signaling pathway, primarily studied in model organisms like *Escherichia coli*. It plays a critical role in coordinating cellular responses to environmental chemical gradients by relaying signals from transmembrane chemoreceptors to flagellar motors. CheA operates as part of a multiprotein complex at the cell pole, interacting with chemoreceptors (e.g., Tar, Tsr) and the adaptor protein CheW. Upon ligand binding to receptors, CheA autophosphorylates at a conserved histidine residue (His48), initiating a phosphorelay cascade that ultimately regulates swimming behavior by modulating flagellar rotation.
Recombinant CheA protein is engineered for *in vitro* studies to dissect its structure-function relationships, enzymatic activity, and interaction networks. Produced via heterologous expression in *E. coli*, it is often purified using affinity tags (e.g., His-tag) for convenience. Researchers utilize recombinant CheA to investigate kinase regulation, ATP-binding kinetics, and phosphorylation dynamics. Mutagenesis studies with recombinant variants have identified key residues involved in catalytic activity, dimerization, and receptor binding. Additionally, its truncated domains (e.g., P1 for phosphorylation, P4/P5 for dimerization) are employed to study modular functions.
Applications extend to structural biology (crystallography, NMR) and drug discovery, particularly in targeting bacterial signaling pathways. CheA’s conservation across pathogens also makes it a candidate for antimicrobial strategies. Overall, recombinant CheA serves as a vital tool for unraveling bacterial motility mechanisms and developing anti-infective therapies.
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