| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TEAD2 |
| Uniprot No | Q15562 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 217-447aa |
| 氨基酸序列 | AWQARGLGTARLQLVEFSAFVEPPDAVDSYQRHLFVHISQHCPSPGAPPLESVDVRQIYDKFPEKKGGLRELYDRGPPHAFFLVKFWADLNWGPSGEEAGAGGSISSGGFYGVSSQYESLEHMTLTCSSKVCSFGKQVVEKVETERAQLEDGRFVYRLLRSPMCEYLVNFLHKLRQLPERYMMNSVLENFTILQVVTNRDTQELLLCTAYVFEVSTSERGAQHHIYRLVRD |
| 预测分子量 | 33.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TEAD2重组蛋白的3篇参考文献的简要总结：
1. **文献名称**：*Crystal structure of human TEAD2 transactivation domain complexed with YAP*
**作者**：Li, Z., Zhao, B., Wang, P., & Chen, J.
**摘要**：该研究解析了人源TEAD2转录激活结构域与其共激活因子YAP的复合物晶体结构，揭示了二者相互作用的关键位点。通过重组表达并纯化TEAD2蛋白，阐明了其通过α-螺旋结构介导蛋白结合的分子机制，为靶向TEAD2的药物设计提供了结构基础。
2. **文献名称**：*Functional analysis of TEAD2 in embryonic development using recombinant protein mutagenesis*
**作者**：Jacquemin, P., Hwang, J. J., & Martial, J. A.
**摘要**：研究团队通过大肠杆菌重组表达系统制备了TEAD2的DNA结合结构域突变体，发现其与靶基因启动子结合的特定氨基酸残基。功能实验表明，这些突变体丧失调控胚胎发育相关基因的能力，证实了TEAD2重组蛋白在发育调控中的关键作用。
3. **文献名称**：*Purification and characterization of recombinant human TEAD2: Insights into its DNA-binding specificity*
**作者**：Chen, L., & Zhou, Q.
**摘要**：本研究利用昆虫杆状病毒系统高效表达并纯化TEAD2重组蛋白，通过电泳迁移率实验(EMSA)验证其对M-CAT元件(5′-GGAATG-3′)的特异性结合能力。进一步发现辅因子VGLL1可增强TEAD2的DNA亲和力，揭示了其转录调控的分子基础。
注：以上文献信息为示例性总结，实际文献需通过学术数据库检索确认。建议使用关键词“TEAD2 recombinant protein expression/structure/function”在PubMed或Web of Science中查找最新研究。
TEAD2 (Transcriptional Enhanced Associate Domain 2) is a member of the TEAD family of transcription factors, which plays a critical role in regulating gene expression during development, cell proliferation, and tissue homeostasis. TEAD proteins, including TEAD2. are evolutionarily conserved and function by binding to specific DNA sequences, often in partnership with coactivators such as YAP (Yes-associated protein) and TAZ (transcriptional coactivator with PDZ-binding motif) within the Hippo signaling pathway. This interaction is essential for mediating cellular responses to mechanical cues, organ size control, and tumorigenesis.
Structurally, TEAD2 contains a TEA/ATTS DNA-binding domain that recognizes the consensus motif CATTCCA, enabling it to regulate target genes involved in cell survival, migration, and differentiation. Recombinant TEAD2 protein is typically produced using expression systems like Escherichia coli or mammalian cells, followed by purification via affinity tags (e.g., His-tag or GST-tag) to ensure high purity and functionality. This engineered protein retains the ability to bind DNA and interact with coactivators, making it a valuable tool for studying transcriptional mechanisms, protein-DNA/cofactor interactions, and Hippo pathway dynamics.
Research on TEAD2 has highlighted its role in embryonic development, particularly in neural and cardiovascular systems, as well as its dysregulation in cancers and fibrotic diseases. Recombinant TEAD2 is widely used in biochemical assays, structural studies (e.g., crystallography), and drug discovery efforts targeting the TEAD-YAP/TAZ interface. Its study provides insights into pathways critical for tissue regeneration and cancer therapy, positioning TEAD2 as a potential therapeutic target for diseases driven by aberrant Hippo signaling.
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