| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PPP2R2B |
| Uniprot No | Q00005 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-443aa |
| 氨基酸序列 | MEEDIDTRKINNSFLRDHSYATEADIISTVEFNHTGELLATGDKGGRVVIFQREQESKNQVHRRGEYNVYSTFQSHEPEFDYLKSLEIEEKINKIRWLPQQNAAYFLLSTNDKTVKLWKVSERDKRPEGYNLKDEEGRLRDPATITTLRVPVLRPMDLMVEATPRRVFANAHTYHINSISVNSDYETYMSADDLRINLWNFEITNQSFNIVDIKPANMEELTEVITAAEFHPHHCNTFVYSSSKGTIRLCDMRASALCDRHTKFFEEPEDPSNRSFFSEIISSISDVKFSHSGRYIMTRDYLTVKVWDLNMENRPIETYQVHDYLRSKLCSLYENDCIFDKFECVWNGSDSVIMTGSYNNFFRMFDRNTKRDVTLEASRENSKPRAILKPRKVCVGGKRRKDEISVDSLDFSKKILHTAWHPSENIIAVAATNNLYIFQDKVN |
| 预测分子量 | 56.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PPP2R2B重组蛋白的参考文献示例，包括文献名称、作者及摘要内容概述：
---
1. **文献名称**：*Structural Insights into the Assembly of the PP2A-B55β Holoenzyme*
**作者**：Y. Huang et al.
**摘要**：本研究通过重组蛋白技术表达了PPP2R2B(B55β亚基)，并利用冷冻电镜解析其与PP2A核心酶复合物的三维结构。结果揭示了B55β的WD40结构域如何介导底物识别及磷酸酶活性调控，为靶向PP2A的药物设计提供了结构基础。
2. **文献名称**：*CAG Repeat Expansion in PPP2R2B Alters Protein Stability and Function in SCA12*
**作者**：J. Smith et al.
**摘要**：通过构建携带异常CAG重复序列的重组PPP2R2B蛋白，研究发现其导致蛋白聚集并破坏与PP2A核心酶的结合能力。这可能是脊髓小脑性共济失调12型(SCA12)中神经元功能失调的关键机制。
3. **文献名称**：*Optimized Expression and Functional Characterization of Recombinant PPP2R2B in E. coli*
**作者**：M. Lee et al.
**摘要**：本文报道了一种高效表达和纯化重组PPP2R2B蛋白的方法，通过优化大肠杆菌表达系统获得高产量活性蛋白。该蛋白成功用于体外磷酸酶活性实验，验证了其对PP2A底物选择性的调控作用。
4. **文献名称**：*PPP2R2B-Mediated Dephosphorylation of AKT Drives Tumor Suppression in Breast Cancer*
**作者**：C. Zhang et al.
**摘要**：利用重组PPP2R2B蛋白的体外实验表明，其通过招募PP2A至AKT信号通路关键节点，促进AKT去磷酸化并抑制肿瘤生长。研究强调了PPP2R2B在癌症中的潜在治疗价值。
---
**注意**：以上文献为示例，实际引用时需核实具体文献来源及准确性。建议通过PubMed、Web of Science等数据库检索最新研究。
**Background of PPP2R2B Recombinant Protein**
PPP2R2B (Protein Phosphatase 2 Regulatory Subunit Bβ) is a gene encoding the B55β regulatory subunit of the serine/threonine protein phosphatase 2A (PP2A) complex. PP2A is a critical enzyme involved in the regulation of diverse cellular processes, including cell cycle progression, signal transduction, and apoptosis. The PP2A holoenzyme consists of a catalytic subunit (C), a structural subunit (A), and a variable regulatory subunit (B). The B subunits, such as PPP2R2B, determine substrate specificity, subcellular localization, and enzymatic activity.
PPP2R2B specifically belongs to the B55/B subfamily and is highly expressed in neuronal tissues. It plays a role in modulating synaptic plasticity, neuronal differentiation, and survival. Dysregulation of PPP2R2B has been implicated in neurodegenerative disorders, such as spinocerebellar ataxia (SCA), and certain cancers. Notably, a CAG repeat expansion in the PPP2R2B gene is linked to autosomal dominant spinocerebellar ataxia type 12 (SCA12), a rare neurological disorder characterized by motor and cognitive deficits.
Recombinant PPP2R2B protein is produced using molecular cloning techniques, often expressed in bacterial or mammalian systems to ensure proper folding and post-translational modifications. This recombinant form enables researchers to study PP2A’s regulatory mechanisms, interactions with binding partners, and its role in disease pathways. It serves as a vital tool for *in vitro* assays, structural studies, and drug discovery efforts targeting PP2A-associated disorders.
Understanding PPP2R2B’s molecular functions and pathological contributions through recombinant protein studies may offer insights into therapeutic strategies for neurological diseases and cancer.
×
