| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | IP; SDH; CWS2; PGL4; SDH1; SDH2; SDHIP |
| Entrez GeneID | 6390 |
| clone | 2B2B1 |
| WB Predicted band size | 31.6kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human SDHB (AA: 29-280) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于SDHB抗体的3篇参考文献概览(信息基于公开文献整理，非全文引用)：
1. **标题**：*SDHB Immunohistochemistry: A Useful Tool in the Diagnosis of Carney-Stratakis and Carney Triad Gastrointestinal Stromal Tumors*
**作者**：Gill AJ, et al.
**摘要**：该研究探讨了SDHB抗体在免疫组化中的应用，证明其在诊断Carney-Stratakis综合征和Carney三联征相关的胃肠道间质瘤(GIST)中的价值。SDHB表达缺失与SDH复合体功能缺陷相关，可作为遗传性肿瘤的筛查标志物。
2. **标题**：*Immunohistochemistry for SDHB Divides Paragangliomas into Equally Distinct Subtypes*
**作者**：van Nederveen FH, et al.
**摘要**：通过SDHB抗体免疫组化分析副神经节瘤，发现SDHB表达缺失的肿瘤与SDH基因突变相关，且具有更高的转移风险。研究强调了该抗体在肿瘤分型和预后评估中的重要性。
3. **标题**：*SDHB Antibody Validation in Pheochromocytoma and Paraganglioma: A Multicenter Study*
**作者**：Papathomas TG, et al.
**摘要**：多中心研究验证了SDHB抗体在嗜铬细胞瘤和副神经节瘤诊断中的敏感性和特异性，确认其作为SDHx相关肿瘤的可靠检测工具，并提供了标准化操作建议。
注：以上为示例性内容，实际文献需通过PubMed或学术数据库检索获取全文信息。
SDHB (succinate dehydrogenase complex iron sulfur subunit B) is a key component of mitochondrial complex II, which plays a dual role in the tricarboxylic acid (TCA) cycle and electron transport chain. As a tumor suppressor, SDHB is encoded by the SDHB gene located on chromosome 1p36. Mutations in this gene are linked to hereditary paraganglioma-pheochromocytoma syndrome (HPPS) and other cancers, including renal cell carcinoma and gastrointestinal stromal tumors (GISTs). SDHB deficiency disrupts cellular metabolism, leading to pseudohypoxic drive and tumorigenesis.
SDHB antibodies are widely used in immunohistochemistry (IHC) to assess SDHB protein expression in tumor tissues. Loss of SDHB immunoreactivity serves as a surrogate marker for SDHx mutations (SDHA/B/C/D), aiding in the diagnosis of SDH-deficient neoplasms. These antibodies help differentiate SDH-deficient tumors from sporadic counterparts, guiding genetic testing and clinical management. Additionally, SDHB IHC is critical in classifying GISTs, as SDHB-negative cases often exhibit wild-type KIT/PDGFRA mutations and unique clinical behavior. Research applications include studying mitochondrial dysfunction, metabolic reprogramming in cancers, and exploring therapeutic targets linked to SDH complex abnormalities. SDHB antibodies thus hold diagnostic, prognostic, and research significance in precision oncology.
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