| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KRT24 |
| Uniprot No | Q2M2I5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-525aa |
| 氨基酸序列 | MSCSSRASSSRAGGSSSARVSAGGSSFSSGSRCGLGGSSAQGFRGGASSCSLSGGSSGAFGGSFGGGFGSCSVGGGFGGASGSGTGFGGGSSFGGVSGFGRGSGFCGSSRFSSGATGGFYSYGGGMGGGVGDGGLFSGGEKQTMQNLNDRLANYLDKVRALEEANTDLENKIKEWYDKYGPGSGDGGSGRDYSKYYSIIEDLRNQIIAATVENAGIILHIDNARLAADDFRLKYENELCLRQSVEADINGLRKVLDDLTMTRSDLEMQIESFTEELAYLRKNHEEEMKNMQGSSGGEVTVEMNAAPGTDLTKLLNDMRAQYEELAEQNRREAEERFNKQSASLQAQISTDAGAATSAKNEITELKRTLQALEIELQSQLAMKSSLEGTLADTEAGYVAQLSEIQTQISALEEEICQIWGETKCQNAEYKQLLDIKTRLEVEIETYRRLLDGEGGGSSFAEFGGRNSGSVNMGSRDLVSGDSRSGSCSGQGRDSSKTRVTKTIVEELVDGKVVSSQVSSISEVKVK |
| 预测分子量 | 57.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于KRT24重组蛋白的3篇虚构参考文献示例(基于研究领域常见主题推测，实际文献需通过学术数据库检索确认)：
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1. **文献名称**: *"Recombinant Expression and Functional Characterization of Human Keratin 24 (KRT24) in Epidermal Differentiation"*
**作者**: Smith J, et al.
**摘要**: 本研究成功构建了KRT24的重组表达系统，利用大肠杆菌表达并纯化KRT24蛋白。实验表明重组KRT24在体外模型中参与调节表皮细胞分化，并可能与细胞骨架重塑相关。
2. **文献名称**: *"KRT24 Recombinant Protein Interacts with Filaggrin in Skin Barrier Formation: Implications for Ichthyosis Pathogenesis"*
**作者**: Lee S, et al.
**摘要**: 通过哺乳动物细胞系表达KRT24重组蛋白，发现其与丝聚蛋白(Filaggrin)存在直接相互作用，提示KRT24在皮肤屏障功能中的关键作用，为先天性鱼鳞病的分子机制提供了新见解。
3. **文献名称**: *"Optimization of Keratin 24 Recombinant Production in Pichia pastoris for Biomedical Applications"*
**作者**: García R, et al.
**摘要**: 研究通过毕赤酵母系统优化KRT24重组蛋白的高效表达与纯化工艺，验证其生物相容性，为未来开发基于角蛋白的组织工程材料奠定基础。
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如需真实文献，建议在PubMed或Web of Science中检索关键词：**"KRT24 recombinant protein"** 或 **"Keratin 24 expression"**，并筛选近年发表的研究。
Keratins are a diverse family of intermediate filament proteins essential for maintaining the structural integrity and mechanical stability of epithelial cells. The KRT24 gene encodes keratin 24. a type I acidic keratin primarily expressed in specific epithelial tissues. While the functional roles of many keratins in skin, hair, and mucosal tissues are well-characterized, KRT24 remains less studied, with emerging evidence linking it to cellular stress responses, differentiation, and tissue repair processes. Its expression has been detected in the skin, hair follicles, and certain internal epithelia, suggesting potential roles in barrier function and regenerative pathways.
Recombinant KRT24 protein is produced using biotechnological platforms, such as bacterial (E. coli), yeast, or mammalian expression systems, to enable large-scale synthesis. These systems are optimized to ensure proper folding, post-translational modifications (when applicable), and solubility. Purification techniques like affinity chromatography or tag-based methods are employed to achieve high-purity protein batches. Recombinant KRT24 retains biological activity, allowing researchers to study its interactions with cytoskeletal components, signaling molecules, and extracellular matrices in vitro.
Current applications of recombinant KRT24 span basic research and translational studies. It is used to investigate keratin-mediated cellular resilience against mechanical stress, oxidative damage, or pathogenic insults. In disease modeling, it aids in exploring keratin-related disorders, such as epithelial fragility syndromes or inflammatory skin conditions. Additionally, recombinant KRT24 serves as an antigen for antibody development, facilitating diagnostic assays or targeted therapies. Emerging interest in tissue engineering also highlights its potential in designing biomimetic scaffolds for epithelial regeneration. Further research is needed to fully elucidate its physiological and pathological significance.
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