| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NOTUM |
| Uniprot No | Q6P988 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 20-496aa |
| 氨基酸序列 | SEGRKTWRRRGQQPPPPPRTEAAPAAGQPVESFPLDFTAVEGNMDSFMAQVKSLAQSLYPCSAQQLNEDLRLHLLLNTSVTCNDGSPAGYYLKESRGSRRWLLFLEGGWYCFNRENCDSRYDTMRRLMSSRDWPRTRTGTGILSSQPEENPYWWNANMVFIPYCSSDVWSGASSKSEKNEYAFMGALIIQEVVRELLGRGLSGAKVLLLAGSSAGGTGVLLNVDRVAEQLEKLGYPAIQVRGLADSGWFLDNKQYRHTDCVDTITCAPTEAIRRGIRYWNGVVPERCRRQFQEGEEWNCFFGYKVYPTLRCPVFVVQWLFDEAQLTVDNVHLTGQPVQEGLRLYIQNLGRELRHTLKDVPASFAPACLSHEIIIRSHWTDVQVKGTSLPRALHCWDRSLHDSHKASKTPLKGCPVHLVDSCPWPHCNPSCPTVRDQFTGQEMNVAQFLMHMGFDMQTVAQPQGLEPSELLGMLSNGS |
| 预测分子量 | 57.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NOTUM重组蛋白的3篇参考文献及简要摘要：
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1. **文献名称**：*Structural basis of Wnt inhibition by Notum*
**作者**：Kakugawa S. et al.
**摘要**：本研究解析了人类NOTUM重组蛋白的晶体结构，揭示了其通过水解Wnt蛋白棕榈酰化修饰抑制Wnt信号通路的分子机制，为靶向NOTUM的药物设计提供了结构基础。
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2. **文献名称**：*Notum suppresses the oncogenic signaling of Wnts in colon cancer*
**作者**：Zhang X. et al.
**摘要**：通过重组NOTUM蛋白实验，研究发现其在结肠癌细胞中通过去棕榈酰化抑制Wnt/β-catenin信号通路，显著降低肿瘤生长，提示其作为潜在治疗靶点。
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3. **文献名称**：*Recombinant expression and enzymatic characterization of human NOTUM carboxylesterase*
**作者**：Jiang Y. et al.
**摘要**：报道了人源NOTUM重组蛋白在大肠杆菌中的高效表达与纯化方法，并验证其对Wnt3a蛋白的去棕榈酰化活性，为后续功能研究提供工具。
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(注：以上文献信息为模拟示例，实际引用时请核对原文准确性。)
NOTUM is a secreted carboxylesterase that belongs to the α/β-hydrolase superfamily. It was initially identified as a negative regulator of the Wnt signaling pathway, a critical pathway involved in embryonic development, tissue homeostasis, and cancer progression. NOTUM exerts its function by removing acetyl groups from glypicans, a family of glycosylphosphatidylinositol (GPI)-anchored heparan sulfate proteoglycans. This deacetylation activity disrupts the ability of glypicans to facilitate Wnt ligand presentation to receptors, thereby attenuating Wnt signaling. Dysregulation of NOTUM has been implicated in various diseases, including colorectal cancer, osteoporosis, and neurodegenerative disorders, making it a potential therapeutic target.
Structurally, NOTUM contains a catalytic triad (Ser-His-Asp) common to serine hydrolases, which is essential for its enzymatic activity. Its substrate specificity and mechanism have been elucidated through crystallography, revealing interactions with glypican core proteins. Recent studies also suggest context-dependent roles for NOTUM beyond Wnt modulation, such as influencing lipid metabolism and extracellular matrix remodeling.
Recombinant NOTUM protein is typically produced using mammalian expression systems (e.g., HEK293 or CHO cells) to ensure proper post-translational modifications and secretion. It serves as a vital tool for biochemical assays, inhibitor screening, and structural studies. Researchers utilize it to investigate Wnt-pathway dynamics, develop small-molecule inhibitors for cancer therapy, and explore its involvement in tissue regeneration. The development of active-site inhibitors and monoclonal antibodies targeting NOTUM has gained momentum, highlighting its translational relevance in restoring Wnt signaling in pathological conditions.
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