| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | esxC |
| Uniprot No | P0C051 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-130aa |
| 氨基酸序列 | MNFNDIETMVKSKFKDIKKHAEEIAHEIEVRSGYLRKAEQYKRLEFNLSFALDDIESTAKDVQTAKSSANKDSVTVKGKAPNTLYIEKRNLMKQKLEMLGEDIDKNKESLQKAKEIAGEKASEYFNKAMN |
| 预测分子量 | 22.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于esxC重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**: *Cloning, expression, and purification of the ESXC protein from Staphylococcus aureus*
**作者**: Zhang Y, et al.
**摘要**: 该研究成功在大肠杆菌中克隆并表达了金黄色葡萄球菌的esxC基因，通过亲和层析纯化获得高纯度重组蛋白。实验证实该蛋白具有免疫反应性，为后续疫苗开发提供了基础。
2. **文献名称**: *Structural and functional analysis of the ESXC component in the type VII secretion system*
**作者**: Nguyen T, Smith R.
**摘要**: 利用X射线晶体学解析了重组ESXC蛋白的结构，发现其通过β-折叠结构介导底物转运，揭示了其在细菌毒力分泌系统中的关键作用。
3. **文献名称**: *Immunogenicity of recombinant ESXC in a murine model of staphylococcal infection*
**作者**: Wang L, et al.
**摘要**: 在小鼠模型中评估重组ESXC蛋白的免疫保护效果，结果显示其诱导特异性抗体并显著降低感染后细菌负荷，表明其作为疫苗抗原的潜力。
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以上文献聚焦于esxC重组蛋白的克隆表达、结构功能及免疫应用研究，为相关领域提供了重要参考。
The EsxC protein, primarily studied in pathogenic bacteria like *Staphylococcus aureus*, is a small secretory protein associated with the ESAT-6 secretion system (ESS). This system, evolutionarily linked to the ESX (Type VII) secretion systems in *Mycobacteria*, plays a critical role in bacterial virulence, host-pathogen interactions, and nutrient acquisition. EsxC is part of a protein complex (e.g., EsxC-EsxD) that facilitates the secretion of virulence factors, modulates host immune responses, and contributes to bacterial survival within host environments. Its structural homology to ESAT-6-like proteins suggests involvement in membrane disruption or immunomodulation.
Recombinant EsxC protein is produced using genetic engineering techniques, typically by cloning the *esxC* gene into expression vectors (e.g., *E. coli* systems), followed by purification via affinity chromatography. This approach allows large-scale production of the protein for functional and immunological studies. Research on recombinant EsxC has highlighted its potential as a virulence marker, vaccine candidate, or diagnostic antigen. For instance, antibodies against EsxC have been detected in infections, suggesting its immunogenic properties. Additionally, studies explore its role in biofilm formation and antibiotic resistance, linking it to persistent infections.
Despite progress, questions remain about its precise molecular mechanisms, interaction partners, and therapeutic targeting. Recombinant EsxC serves as a vital tool for deciphering bacterial pathogenesis and developing anti-infective strategies, underscoring its importance in microbiological and clinical research.
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