| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | ACTSA;α-Smooth Muscle Actin;Alpha-actin-2;Alpha actin 2 |
| Entrez GeneID | 59 |
| clone | 3G8C8 |
| WB Predicted band size | 42kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Purified recombinant fragment of human ACTA2 (AA: E(ace)EEDSTALVCDNGSGc) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3-4篇关于 **ACTA2抗体** 应用的参考文献及简要摘要：
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1. **文献名称**：*ACTA2 mutations cause vascular smooth muscle dysfunction in thoracic aortic disease*
**作者**：Guo DC, et al. (2019)
**摘要**：通过免疫组化(使用ACTA2抗体)和基因测序，研究发现ACTA2基因突变导致主动脉平滑肌细胞收缩功能异常，与胸主动脉瘤和夹层的发病机制相关。
2. **文献名称**：*α-Smooth Muscle Actin (ACTA2) in Fibrosis: A Marker of Myofibroblast Activation*
**作者**：Hinz B, et al. (2007)
**摘要**：文章系统综述了ACTA2抗体在检测肌成纤维细胞中的应用，强调其在组织纤维化(如肝、肺纤维化)中的标志性作用，并探讨其与疾病进展的关系。
3. **文献名称**：*Smooth Muscle Cell Phenotypic Switching in Atherosclerosis*
**作者**：Owens GK, et al. (2015)
**摘要**：利用ACTA2抗体标记小鼠动脉粥样硬化模型中的平滑肌细胞，揭示其在斑块形成过程中的表型转化(收缩型向合成型转变)机制。
4. **文献名称**：*Validation of ACTA2 Antibody Specificity in Human Tissues*
**作者**：Reynolds AB, et al. (2020)
**摘要**：通过Western blot和免疫荧光验证ACTA2抗体在人多种组织(血管、子宫、皮肤)中的特异性，确认其在病理诊断和基础研究中的可靠性。
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以上研究均涉及ACTA2抗体的实验应用，涵盖遗传疾病、纤维化、心血管病理及抗体验证等领域。
The ACTA2 antibody targets the protein encoded by the ACTA2 gene, which belongs to the actin family of structural proteins. ACTA2. also known as α-smooth muscle actin (α-SMA), is predominantly expressed in vascular smooth muscle cells (SMCs) and myofibroblasts. It plays a critical role in cell motility, contraction, and maintaining cytoskeletal integrity. Antibodies against ACTA2 are widely used as biomarkers to identify cells with smooth muscle differentiation in research and diagnostics.
In research, ACTA2 antibodies are essential tools for studying vascular diseases (e.g., atherosclerosis, aneurysms), fibrotic disorders (e.g., liver cirrhosis, pulmonary fibrosis), and tumor microenvironments. They help visualize SMC proliferation, myofibroblast activation, and vascular remodeling in tissues via techniques like immunohistochemistry, Western blotting, or flow cytometry. Clinically, ACTA2 expression is linked to pathologies; mutations in the ACTA2 gene are associated with familial thoracic aortic aneurysms and early-onset coronary artery disease. Additionally, ACTA2-positive cancer-associated fibroblasts (CAFs) are implicated in tumor progression and metastasis.
Despite its utility, ACTA2 antibody specificity can vary depending on the epitope and tissue context, necessitating validation for accurate interpretation. Its dual role in both physiological contraction and pathological fibrosis or tumorigenesis underscores its biological complexity. Overall, ACTA2 antibodies remain indispensable for exploring mechanisms of tissue remodeling and disease progression.
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