| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CCDC88A |
| Uniprot No | Q3V6T2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1713-1823aa |
| 氨基酸序列 | SLSVSSDFLGKDKPVSCGLARSVSGKTPGDFYDRRTTKPEFLRPGPRKTEDTYFISSAGKPTPGTQGKIKLVKESSLSRQSKDSNPYATLPRASSVISTAEGTTRRTSIHD |
| 预测分子量 | 16.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CCDC88A重组蛋白的模拟参考文献示例(仅供参考，非真实文献)：
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1. **文献名称**：*CCDC88A重组蛋白在Wnt信号通路中的支架作用研究*
**作者**：Zhang L, et al.
**摘要**：研究通过大肠杆菌表达系统制备CCDC88A重组蛋白，证实其作为支架蛋白与β-catenin相互作用，调控Wnt信号通路活性，影响结肠癌细胞增殖。
2. **文献名称**：*CCDC88A重组蛋白的晶体结构解析及功能域分析*
**作者**：Müller R, et al.
**摘要**：利用重组CCDC88A蛋白进行X射线晶体学研究，揭示其N端卷曲螺旋结构域的关键作用，为探究其在细胞极性调控中的分子机制提供结构基础。
3. **文献名称**：*CCDC88A重组蛋白在肝纤维化中的免疫调控功能*
**作者**：Wang Y, et al.
**摘要**：通过哺乳动物细胞表达纯化CCDC88A重组蛋白，发现其与TGF-β1受体结合，激活肝星状细胞促纤维化通路，提示其作为肝病治疗潜在靶点。
4. **文献名称**：*CCDC88A重组蛋白与Dvl2的相互作用及其在细胞迁移中的作用*
**作者**：Saito K, et al.
**摘要**：采用GST pull-down实验验证重组CCDC88A与Dishevelled-2(Dvl2)的直接结合，阐明其通过调控细胞骨架重排影响乳腺癌细胞迁移能力。
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注：以上内容为示例性模拟，实际文献需通过学术数据库(如PubMed、Web of Science)检索。
CCDC88A recombinant protein is derived from the CCDC88A gene, which encodes a scaffolding protein known for its role in regulating diverse cellular processes, including signal transduction, cytoskeletal organization, and cell migration. The protein, also termed Girders of Actin Filaments (GIV) or Hook-related protein 2 (HkRP2), contains multiple functional domains, such as a central helical domain and a C-terminal Gα-binding motif, enabling interactions with signaling molecules like G proteins, growth factor receptors, and cytoskeletal components. Its involvement in pathways such as Wnt/β-catenin and TGF-β underscores its significance in development, tissue homeostasis, and disease contexts like cancer and fibrosis.
Recombinant CCDC88A is engineered using expression systems (e.g., E. coli, mammalian cells) to produce purified, biologically active protein for functional studies. These proteins often include affinity tags (e.g., His, GST) to facilitate purification and detection. Researchers utilize recombinant CCDC88A to investigate its molecular mechanisms, such as its dual role as a guanine nucleotide exchange factor (GEF) for Gαi subunits and a modulator of receptor tyrosine kinase signaling. Its dysregulation has been linked to pathological conditions, including tumor metastasis, immune dysregulation, and organ fibrosis, making it a potential therapeutic target.
Studies employing recombinant CCDC88A have clarified its role in cell polarity, migration, and invasion, particularly in cancer cells. Additionally, its interaction with cytoskeletal proteins highlights its influence on cell morphology and mechanotransduction. The recombinant form enables in vitro assays, structural analyses, and antibody development, aiding biomarker discovery and drug screening. Ongoing research focuses on its tissue-specific functions and therapeutic modulation in diseases characterized by aberrant cell signaling or motility.
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