| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | FCC2; COCA2; HNPCC; hMLH1; HNPCC2 |
| Entrez GeneID | 4292 |
| clone | 5A3F6 |
| WB Predicted band size | 84.6kDa |
| Host/Isotype | Mouse IgG2b |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human MLH1 (AA: 381-483) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3条与MLH1抗体相关的文献摘要概括：
1. **文献名称**: *"MLH1 Immunohistochemistry in Colorectal Cancer: A Practical Review"*
**作者**: Watson N, et al.
**摘要**: 本文综述了MLH1抗体在结直肠癌免疫组化(IHC)中的应用，强调其在检测错配修复缺陷(dMMR)中的重要性。研究比较了不同克隆号抗体的敏感性和特异性，并讨论了MLH1缺失与散发性癌症及林奇综合征的关联。
2. **文献名称**: *"Comparative Analysis of MLH1 and PMS2 Antibodies for Lynch Syndrome Screening"*
**作者**: Rodriguez-Bigas MA, et al.
**摘要**: 该研究评估了MLH1抗体(克隆G168-15)与PMS2抗体联合使用的效果，发现MLH1蛋白表达缺失常伴随PMS2表达异常，建议联合检测以提高林奇综合征筛查的准确性，并验证了抗体在福尔马林固定组织中的稳定性。
3. **文献名称**: *"Automated MLH1 Methylation Analysis in Endometrial Carcinoma Using MLH1 Antibody-Positive Selection"*
**作者**: Haraldsdottir S, et al.
**摘要**: 研究提出一种结合MLH1抗体(克隆M1)免疫组化与自动化甲基化检测的方法，用于区分MLH1启动子甲基化导致的散发性子宫内膜癌与非甲基化的林奇综合征相关病例，验证了抗体在分子分型中的辅助价值。
The MLH1 antibody is a crucial tool in molecular pathology and cancer research, targeting the MLH1 protein encoded by the *MLH1* gene. This protein is a key component of the DNA mismatch repair (MMR) system, which corrects replication errors and maintains genomic stability. MLH1 forms heterodimers with PMS2. creating the MutLα complex that coordinates mismatch recognition and repair. Defects in MLH1. often due to mutations or epigenetic silencing, disrupt MMR function, leading to microsatellite instability (MSI) and an elevated risk of cancers, particularly colorectal, endometrial, and gastric cancers.
In clinical diagnostics, MLH1 antibodies are widely used in immunohistochemistry (IHC) to assess MLH1 protein expression in tumor tissues. Loss of MLH1 expression is a hallmark of Lynch syndrome (hereditary non-polyposis colorectal cancer) and sporadic cancers with MLH1 promoter hypermethylation. This antibody aids in identifying patients who may benefit from genetic testing or immunotherapy, as MSI-high tumors often respond better to immune checkpoint inhibitors.
In research, MLH1 antibodies facilitate studies on DNA repair mechanisms, carcinogenesis, and therapy resistance. They are also used to validate MLH1 knockout models and investigate epigenetic regulation. Commercially available monoclonal antibodies are optimized for specificity in formalin-fixed, paraffin-embedded tissues, ensuring reliability in both diagnostic and experimental applications. Understanding MLH1's role through antibody-based assays continues to advance precision oncology and hereditary cancer management.
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