| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Rnaset2a |
| Uniprot No | C0HKG5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 30-259aa |
| 氨基酸序列 | GPLWSGSHEWKKLILTQHWPPTVCKEVNSCQDSLDYWTIHGLWPDRAEDCNQSWHFNLDEIKDLLRDMKIYWPDVIHRSSNRSQFWKHEWVKHGTCAAQVDALNSEKKYFGKSLDLYKQIDLNSVLQKFGIKPSINYYQLADFKDALTRIYGVVPKIQCLMPEQGESVQTVGQIELCFTKEDLHLRNCTEPGEQLSSRQEAWLAMEASTHGMMVCEDGPIFYPPPTKTQH |
| 预测分子量 | 34.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于Rnaset2a重组蛋白的3篇参考文献示例(内容为虚构，供参考)：
1. **文献名称**: "Expression and Functional Characterization of Recombinant Rnase T2a in Cancer Models"
**作者**: Smith J, et al.
**摘要**: 研究报道了Rnaset2a重组蛋白在哺乳动物细胞中的表达与纯化，并发现其通过降解肿瘤微环境中特定RNA分子抑制血管生成，提示其潜在抗肿瘤作用。
2. **文献名称**: "Structural Insights into Rnase T2a’s Substrate Specificity"
**作者**: Lee H, Park S.
**摘要**: 通过X射线晶体学解析Rnaset2a重组蛋白的三维结构，揭示其催化活性中心的独特构象，阐明了其对单链RNA底物的特异性切割机制。
3. **文献名称**: "Rnase T2a Recombinant Protein Attenuates Neuroinflammation in Murine Models"
**作者**: García-Ruiz C, et al.
**摘要**: 利用原核系统成功制备高活性Rnaset2a重组蛋白，实验表明其可通过降解炎症相关RNA分子缓解小鼠神经炎症反应，为治疗神经退行性疾病提供新思路。
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**提示**：若需真实文献，建议通过PubMed或Google Scholar检索关键词 **"Rnase T2a recombinant protein"** 或 **"RNASET2 expression and function"**，并筛选近年发表的生化或医学类研究。
**Background of RNase T2a Recombinant Protein**
RNase T2a belongs to the ribonuclease T2 family, a group of endoribonucleases conserved across eukaryotes and involved in RNA metabolism. These enzymes catalyze the cleavage of single-stranded RNA via a conserved acid-base mechanism, typically targeting specific sequences or structures. RNase T2a is characterized by its substrate preference for uridine-rich regions and its role in diverse biological processes, including ribosomal RNA (rRNA) processing, RNA interference, and stress response pathways. Structurally, it shares a conserved catalytic domain with two catalytic aspartate residues critical for enzymatic activity.
The recombinant RNase T2a protein is produced using genetic engineering techniques, often expressed in *E. coli* or mammalian cell systems to ensure proper folding and post-translational modifications. Its recombinant form enables controlled study of enzymatic properties, substrate specificity, and interactions with cellular components. Research has linked RNase T2 family members to immune regulation, tumor suppression, and neurodegenerative diseases, making recombinant variants valuable tools for dissecting their roles in health and disease.
In plants, RNase T2 homologs participate in phosphate recycling and pathogen defense, while in mammals, they contribute to lysosomal RNA degradation and inflammatory responses. Dysregulation of RNase T2a activity has been implicated in pathologies such as cancer progression and autoimmune disorders. Recombinant RNase T2a is thus pivotal for drug discovery, structural studies, and therapeutic applications, offering insights into RNA-mediated cellular mechanisms and potential clinical interventions.
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