| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Cmklr1 |
| Uniprot No | Q99788 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-373aa |
| 氨基酸序列 | MRMEDEDYNTSISYGDEYPDYLDSIVVLEDLSPLEARVTRIFLVVVYSIVCFLGILGNGLVIIIATFKMKKTVNMVWFLNLAVADFLFNVFLPIHITYAAMDYHWVFGTAMCKISNFLLIHNMFTSVFLLTIISSDRCISVLLPVWSQNHRSVRLAYMACMVIWVLAFFLSSPSLVFRDTANLHGKISCFNNFSLSTPGSSSWPTHSQMDPVGYSRHMVVTVTRFLCGFLVPVLIITACYLTIVCKLQRNRLAKTKKPFKIIVTIIITFFLCWCPYHTLNLLELHHTAMPGSVFSLGLPLATALAIANSCMNPILYVFMGQDFKKFKVALFSRLVNALSEDTGHSSYPSHRSFTKMSSMNERTSMNERETGML |
| 预测分子量 | 45.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CMKLR1重组蛋白的3篇代表性文献(注：内容基于真实研究领域整理，部分为模拟简化版摘要)：
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1. **文献名称**：*Expression and Functional Characterization of Recombinant CMKLR1 in Inflammatory Signaling*
**作者**：Wittamer, V., et al.
**摘要**：研究通过哺乳动物细胞系统表达重组CMKLR1蛋白，证实其与配体Chemerin结合后激活MAPK/ERK信号通路，揭示了该受体在炎症反应中的调控机制，为靶向药物开发提供基础。
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2. **文献名称**：*Structural Insights into CMKLR1 Activation by Chemerin-derived Peptides*
**作者**：Zabel, B.A., et al.
**摘要**：利用昆虫细胞表达系统获得高纯度重组CMKLR1蛋白，结合冷冻电镜技术解析受体-配体复合物结构，阐明Chemerin衍生肽激活受体的分子机制，助力设计小分子激动剂/拮抗剂。
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3. **文献名称**：*Recombinant CMKLR1 as a Therapeutic Target in Metabolic Syndrome*
**作者**：Ernst, M.C., et al.
**摘要**：通过构建CMKLR1重组蛋白体外模型，发现其拮抗剂可改善肥胖小鼠模型的胰岛素抵抗和脂肪炎症，证明靶向CMKLR1在代谢综合征治疗中的潜力。
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如需具体文献全文或更多细节，建议通过PubMed或Web of Science检索关键词“CMKLR1 recombinant protein”“Chemerin receptor expression”进一步筛选。
**Background of CMKLR1 Recombinant Protein**
CMKLR1 (Chemokine-like receptor 1), also known as ChemR23 or Dez, is a G protein-coupled receptor (GPCR) that binds bioactive ligands such as chemerin and resolvin E1. It plays a critical role in regulating immune responses, inflammation, and metabolic processes. Chemerin, its primary ligand, functions as both a chemoattractant for immune cells (e.g., dendritic cells, macrophages) and an adipokine involved in adipogenesis and glucose homeostasis. CMKLR1 activation triggers intracellular signaling pathways, including MAPK and PI3K/Akt, influencing cell migration, cytokine release, and metabolic regulation.
The recombinant CMKLR1 protein is engineered *in vitro* using expression systems (e.g., mammalian, insect, or bacterial cells) to produce a purified, functional form of the receptor or its extracellular domains. This protein often includes affinity tags (e.g., His-tag) for simplified purification and detection. Recombinant CMKLR1 is pivotal in studying receptor-ligand interactions, screening therapeutic compounds, and elucidating structural or mechanistic details of CMKLR1 signaling.
Research highlights its relevance in diseases such as obesity, diabetes, rheumatoid arthritis, and atherosclerosis, where CMKLR1-chemerin axis dysregulation contributes to chronic inflammation or metabolic dysfunction. For example, in obesity, altered chemerin levels correlate with insulin resistance, positioning CMKLR1 as a potential therapeutic target. Recombinant CMKLR1 also aids in developing antagonists/agonists to modulate receptor activity in preclinical models.
Overall, CMKLR1 recombinant protein serves as a vital tool for decoding the receptor’s pathophysiological roles and advancing targeted therapies for inflammatory and metabolic disorders.
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