| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ABCD2 |
| Uniprot No | Q9UBJ2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-740aa |
| 氨基酸序列 | MTHMLNAAADRVKWTRSSAAKRAACLVAAAYALKTLYPIIGKRLKQSGHGKKKAAAYPAAENTEILHCTETICEKPSPGVNADFFKQLLELRKILFPKLVTTETGWLCLHSVALISRTFLSIYVAGLDGKIVKSIVEKKPRTFIIKLIKWLMIAIPATFVNSAIRYLECKLALAFRTRLVDHAYETYFTNQTYYKVINMDGRLANPDQSLTEDIMMFSQSVAHLYSNLTKPILDVMLTSYTLIQTATSRGASPIGPTLLAGLVVYATAKVLKACSPKFGKLVAEEAHRKGYLRYVHSRIIANVEEIAFYRGHKVEMKQLQKSYKALADQMNLILSKRLWYIMIEQFLMKYVWSSSGLIMVAIPIITATGFADGEDGQKQVMVSERTEAFTTARNLLASGADAIERIMSSYKEVTELAGYTARVYNMFWVFDEVKRGIYKRTAVIQESESHSKNGAKVELPLSDTLAIKGKVIDVDHGIICENVPIITPAGEVVASRLNFKVEEGMHLLITGPNGCGKSSLFRILSGLWPVYEGVLYKPPPQHMFYIPQRPYMSLGSLRDQVIYPDSVDDMHDKGYTDQDLERILHNVHLYHIVQREGGWDAVMDWKDVLSGGEKQRMGMARMFYHKPKYALLDECTSAVSIDVEGKIFQAAKGAGISLLSITHRPSLWKYHTHLLQFDGEGGWRFEQLDTAIRLTLSEEKQKLESQLAGIPKMQQRLNELCKILGEDSVLKTIKNEDETS |
| 预测分子量 | 89.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ABCD2重组蛋白的3篇参考文献及其摘要概括：
1. **文献名称**：*"Functional characterization of recombinant human ABCD2 transporter in yeast peroxisomes"*
**作者**：Smith J, et al.
**摘要**：本研究利用酵母表达系统成功表达了人源ABCD2重组蛋白，证实其定位至过氧化物酶体膜，并验证其作为超长链脂肪酸(VLCFA)转运体的功能缺失与肾上腺脑白质营养不良(ALD)的相关性。
2. **文献名称**：*"Structural insights into ABCD2-mediated substrate transport by cryo-EM"*
**作者**：Li X, et al.
**摘要**：通过冷冻电镜解析了ABCD2重组蛋白的三维结构，揭示了其ATP结合结构域构象变化机制，阐明了该蛋白在脂质底物跨膜转运中的分子基础，为相关代谢疾病治疗提供新靶点。
3. **文献名称**：*"ABCD2 overexpression ameliorates lipid accumulation in cellular models of X-linked adrenoleukodystrophy"*
**作者**：Garcia M, et al.
**摘要**：构建了哺乳动物细胞表达的ABCD2重组蛋白，证明其过表达可补偿ABCD1缺陷细胞中的VLCFA代谢异常，提示ABCD2基因疗法在ALD治疗中的潜在应用价值。
注：以上文献信息为基于领域知识的模拟，实际文献需通过PubMed/Google Scholar检索确认。建议使用关键词"ABCD2 recombinant protein"+"peroxisome"/"lipid metabolism"查找具体论文。
**Background of ABCD2 Recombinant Protein**
ABCD2 (ATP-binding cassette sub-family D member 2), also known as ALDR (adrenoleukodystrophy-related protein), is a peroxisomal transmembrane protein belonging to the ATP-binding cassette (ABC) transporter superfamily. It plays a critical role in transporting very-long-chain fatty acids (VLCFAs) into peroxisomes for β-oxidation, a process essential for lipid metabolism and cellular energy homeostasis. Mutations in the ABCD2 gene are linked to peroxisomal disorders, such as adrenoleukodystrophy (ALD), a rare neurodegenerative condition characterized by VLCFA accumulation.
Recombinant ABCD2 protein is engineered using biotechnological methods to study its structure, function, and interactions. Typically produced in heterologous expression systems like *E. coli*, yeast, or mammalian cells, the recombinant protein retains key domains: two nucleotide-binding domains (NBDs) for ATP hydrolysis and two transmembrane domains (TMDs) forming the substrate channel. Researchers employ affinity tags (e.g., His-tag) for purification and detection.
Studies on recombinant ABCD2 focus on elucidating its substrate specificity, ATPase activity, and role in lipid metabolism. It serves as a therapeutic target for ALD and related metabolic disorders, with efforts to develop small-molecule activators or gene therapies. Additionally, ABCD2 is explored in cancer research due to its potential involvement in drug resistance and lipid-dependent signaling pathways.
The availability of recombinant ABCD2 has advanced structural studies (e.g., cryo-EM) and high-throughput drug screening, offering insights into peroxisomal transport mechanisms and disease pathology. Its application extends to generating antibodies and diagnostic tools, underscoring its biomedical significance.
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