| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | P2yr13 |
| Uniprot No | Q9D8I2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-377aa |
| 氨基酸序列 | MLGTINTTGMQGFNKSERCPRDTRMTQLLFPVLYTVVFLAGILLNTVALWVFVHIPSNSTFIVYLKNTLVADLIMALMLPFKILSDSHLAPWQLRGFVCTLSSVVFYETMYVGIMMLGLIAFDRFLKIIMPFRKTFVKKTAFAKTVSISVWSLMFFISLPNMILNKEATPSSVKKCASLKSPLGLWWHQVVSHTCQFIFWAVFILMLLFYAVITKKVYNSYRKFRSKDSRHKRLEVKVFIVMAVFFVCFAPLHFVRIPYTYSQTTNKTDCRLENQLFIAKEATLFLATTNICMDPLIYIILCKKFTQKVPCVRWGKARTAGSSEDHHSSQTDNITLA |
| 预测分子量 | 44.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于P2Y13重组蛋白的3篇代表性文献的简要信息(注：部分内容基于领域内典型研究方向的概括，具体文献标题和作者为模拟示例)：
1. **标题**：*"Functional characterization of the human P2Y13 receptor expressed in HEK293 cells: Role in high-density lipoprotein metabolism"*
**作者**：Jacquet, S., Malaval, C., Martinez, L.O., et al.
**摘要**：本研究在HEK293细胞中成功重组表达了人源P2Y13受体，证实其通过G蛋白偶联信号通路调控高密度脂蛋白(HDL)摄取，揭示了其在胆固醇逆转运中的关键作用。
2. **标题**：*"Structural insights into the activation mechanism of P2Y13 receptor via cryo-EM"*
**作者**：Zhang, Y., Liu, X., Zhao, Q., et al.
**摘要**：通过冷冻电镜解析了P2Y13受体与配体结合后的三维结构，阐明了其配体识别和信号转导的分子机制，为靶向药物设计提供了结构基础。
3. **标题**：*"P2Y13 receptor regulates microglial phagocytosis and neuroinflammation in Alzheimer's disease models"*
**作者**：Smith, J., Doe, R., Chen, L., et al.
**摘要**：利用重组P2Y13蛋白进行体外实验，发现其激活可增强小胶质细胞对β-淀粉样蛋白的吞噬能力，提示其在阿尔茨海默病神经炎症中的潜在治疗价值。
**说明**：以上文献为示例性质，实际文献需通过PubMed、Web of Science等平台检索确认。P2Y13研究多聚焦于其在代谢疾病(如动脉粥样硬化)和神经退行性疾病中的作用，重组蛋白技术常用于其信号通路和药物筛选研究。
P2Y13 receptor, a member of the P2Y purinergic receptor family, is a G protein-coupled receptor (GPCR) activated by extracellular nucleotides such as adenosine diphosphate (ADP). It plays critical roles in regulating cellular processes like platelet aggregation, neurotransmission, immune responses, and metabolic homeostasis. The P2Y13 receptor is encoded by the *P2RY13* gene in humans and is predominantly expressed in the brain, spleen, and hematopoietic tissues. Structurally, it features seven transmembrane domains, characteristic of GPCRs, with extracellular loops involved in ligand binding and intracellular regions mediating G protein coupling (typically Gαi/o, inhibiting cAMP production).
Recombinant P2Y13 protein refers to the engineered form of this receptor, produced using heterologous expression systems (e.g., mammalian cells, insect cells, or yeast) for functional and pharmacological studies. Its recombinant expression enables large-scale production, purification, and structural analysis, which are essential for drug discovery. Researchers often tag the protein (e.g., with His or FLAG tags) to facilitate purification via affinity chromatography. Challenges in producing functional P2Y13 include maintaining proper post-translational modifications (e.g., glycosylation) and conformational stability during purification.
Current studies focus on P2Y13's role in diseases such as atherosclerosis, neuroinflammation, and type 2 diabetes, making it a potential therapeutic target. Structural insights from recombinant P2Y13 have aided in designing selective agonists/antagonists. However, its signaling complexity, including interactions with arrestins and other secondary messengers, requires further exploration. Advances in cryo-EM and computational modeling are accelerating mechanistic studies, positioning recombinant P2Y13 as a key tool for unraveling nucleotide signaling pathways and developing precision therapies.
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