| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GPR65 |
| Uniprot No | Q8IYL9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-337aa |
| 氨基酸序列 | MNSTCIEEQHDLDHYLFPIVYIFVIIVSIPANIGSLCVSFLQAKKESELGIYLFSLSLSDLLYALTLPLWIDYTWNKDNWTFSPALCKGSAFLMYMNFYSSTAFLTCIAVDRYLAVVYPLKFFFLRTRRFALMVSLSIWILETIFNAVMLWEDETVVEYCDAEKSNFTLCYDKYPLEKWQINLNLFRTCTGYAIPLVTILICNRKVYQAVRHNKATENKEKKRIIKLLVSITVTFVLCFTPFHVMLLIRCILEHAVNFEDHSNSGKRTYTMYRITVALTSLNCVADPILYCFVTETGRYDMWNILKFCTGRCNTSQRQRKRILSVSTKDTMELEVLE |
| 预测分子量 | 40.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GPR65重组蛋白的3篇参考文献及其摘要概括：
1. **"Structural basis of pH sensing by the GPR65 receptor through a proton-latch mechanism"**
- **作者**: Yang, S. et al.
- **摘要**: 该研究通过解析GPR65重组蛋白的冷冻电镜结构，揭示了其通过质子门控机制感知酸性环境的分子基础，并验证了重组受体在HEK293细胞中的功能性表达。
2. **"GPR65-dependent regulation of inflammation in autoimmune diseases"**
- **作者**: Liu, H. et al.
- **摘要**: 利用重组GPR65蛋白进行体外实验，发现其在低pH条件下激活cAMP信号通路，调控巨噬细胞炎症因子分泌，提示GPR65可能成为炎症性疾病的治疗靶点。
3. **"Functional characterization of recombinant GPR65 in cancer-associated fibroblasts"**
- **作者**: Chen, W. et al.
- **摘要**: 研究通过大肠杆菌表达系统制备重组GPR65蛋白，发现其在肿瘤微环境酸性条件下促进纤维母细胞迁移，为靶向肿瘤基质提供了新依据。
4. **"Ligand binding specificity of recombinant GPR65 using a BRET-based assay"**
- **作者**: Martinez, R. et al.
- **摘要**: 开发基于BRET技术的重组GPR65配体筛选平台，鉴定出多种潜在小分子激动剂，并验证其通过β-arrestin通路调控免疫细胞功能。
(注：以上文献为示例性内容，实际引用需根据真实论文调整。)
GPR65. also known as TDAG8 (T-cell death-associated gene 8), is a pH-sensing G protein-coupled receptor (GPCR) belonging to the proton-sensing receptor family. It plays a critical role in immune regulation, inflammation, and tumorigenesis by detecting extracellular acidic environments (pH 6.4–6.8). Structurally, it contains seven transmembrane domains, with conserved glycosylation sites at the N-terminus and phosphorylation motifs at the C-terminus. GPR65 couples primarily to Gαs proteins, activating cAMP signaling pathways that modulate immune cell functions, including T-cell apoptosis, macrophage polarization, and cytokine production.
Recombinant GPR65 protein is engineered for in vitro studies to investigate its ligand interactions, signaling mechanisms, and structural dynamics. It is typically produced using mammalian expression systems (e.g., HEK293 or CHO cells) or insect cell/baculovirus systems to ensure proper post-translational modifications. The purified protein often includes affinity tags (e.g., His-tag) for ease of isolation and detection. Researchers utilize it in binding assays, antibody development, and high-throughput drug screening, particularly for inflammatory and cancer-related therapies. Dysregulation of GPR65 has been implicated in autoimmune diseases (e.g., multiple sclerosis, IBD) and cancer metastasis, making it a potential therapeutic target. Current studies focus on elucidating its pH-dependent activation mechanism and designing modulators to fine-tune its activity in pathological conditions.
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