| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Nkg7 |
| Uniprot No | Q99PA5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-165aa |
| 氨基酸序列 | MEPCRSLALFAGSLGLTSSLIALTTDFWIVATGPHFSAHSGLWPTSQETQVAGYIHVTQSFCILAVLWGLVSVSFLILSCIPALSAPGRGPLVSTVMAFSAALSILVAMAVYTSMRWSQTPFSQVQTFFSWSFYLGWVSFILFLFAGCLSLGAHCRTRRAEYETL |
| 预测分子量 | 19.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NKG7重组蛋白的3篇参考文献及其摘要概括：
1. **《NKG7 enhances CD8+ T cell cytotoxicity by promoting granzyme B expression and cellular degranulation》**
- **作者**: Smith et al.
- **摘要**: 该研究发现NKG7重组蛋白通过上调颗粒酶B(Granzyme B)的表达并促进T细胞的脱颗粒作用，显著增强CD8+ T细胞对肿瘤细胞的杀伤能力，揭示了其在免疫治疗中的潜在应用价值。
2. **《NKG7 regulates the lytic function of human natural killer cells by modulating cytoskeletal dynamics》**
- **作者**: Chen et al.
- **摘要**: 研究通过体外实验证明，NKG7重组蛋白通过调控细胞骨架重排，促进NK细胞与靶细胞的接触及溶酶体极化，从而提升NK细胞的溶瘤活性，为基于NKG7的免疫疗法提供了机制依据。
3. **《Recombinant NKG7 protein rescues antiviral immunity in NKG7-deficient mice》**
- **作者**: Wang et al.
- **摘要**: 该研究利用NKG7重组蛋白在NKG7基因缺陷小鼠模型中恢复了NK细胞对病毒感染细胞的清除能力，证实了NKG7在抗病毒免疫中的关键作用，并探索了其作为免疫增强剂的可行性。
*注：上述文献为示例，实际引用需根据具体研究补充真实文献信息。建议通过PubMed或Google Scholar检索关键词“NKG7 recombinant protein”获取最新研究。*
NKG7 (Natural Killer Cell Granule Protein 7) is a cytotoxic granule-associated protein predominantly expressed in natural killer (NK) cells and activated CD8+ T lymphocytes. It plays a critical role in modulating immune effector functions, particularly in the regulation of cytotoxic granule exocytosis during target cell killing. Structurally, NKG7 is a small, evolutionarily conserved protein with a hydrophobic domain that facilitates its association with secretory vesicles containing perforin and granzymes. Its interaction with cytoskeletal components and vesicle membranes suggests involvement in granule trafficking or membrane fusion processes during immune synapse formation.
Recombinant NKG7 protein is engineered using expression systems (e.g., E. coli or mammalian cells) to produce purified, biologically active forms for functional studies. Researchers utilize it to investigate NKG7's mechanistic roles in cytotoxicity, immune regulation, and its potential links to autoimmune diseases, viral infections, and cancer immunotherapy. Recent studies highlight NKG7 as a biomarker for cytotoxic lymphocyte activity and a potential therapeutic target. In vitro, recombinant NKG7 enables antibody development, structural analysis, and functional assays to dissect its contributions to immune cell communication and pathogen defense. Its applications extend to exploring novel immunotherapies aimed at enhancing or suppressing cytotoxic responses in disease contexts.
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