| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OPN1MW |
| Uniprot No | P04001 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-364aa |
| 氨基酸序列 | MAQQWSLQRLAGRHPQDSYEDSTQSSIFTYTNSNSTRGPFEGPNYHIAPRWVYHLTSVWMIFVVIASVFTNGLVLAATMKFKKLRHPLNWILVNLAVADLAETVIASTISVVNQVYGYFVLGHPMCVLEGYTVSLCGITGLWSLAIISWERWMVVCKPFGNVRFDAKLAIVGIAFSWIWAAVWTAPPIFGWSRYWPHGLKTSCGPDVFSGSSYPGVQSYMIVLMVTCCITPLSIIVLCYLQVWLAIRAVAKQQKESESTQKAEKEVTRMVVVMVLAFCFCWGPYAFFACFAAANPGYPFHPLMAALPAFFAKSATIYNPVIYVFMNRQFRNCILQLFGKKVDDGSELSSASKTEVSSVSSVSPA |
| 预测分子量 | 46.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于OPN1MW重组蛋白的参考文献及其摘要概述：
1. **"Expression and spectral tuning of human cone pigments"**
- **作者**: Oprian, D.D., et al.
- **摘要**: 该研究在HEK293细胞中成功表达了人视锥细胞色素OPN1MW的重组蛋白，并通过光谱分析验证了其对中波(绿色)光的吸收特性，为后续功能研究提供了技术基础。
2. **"Molecular genetics of human color vision: the genes encoding blue, green, and red pigments"**
- **作者**: Nathans, J., et al.
- **摘要**: 本文首次克隆并测序了人视蛋白基因(包括OPN1MW)，揭示了其编码的氨基酸序列差异如何影响光谱敏感性，为重组蛋白的体外表达奠定理论基础。
3. **"Mechanisms of spectral tuning in human red/green cone pigments"**
- **作者**: Shichida, Y., et al.
- **摘要**: 通过重组表达OPN1MW及其突变体，研究团队发现特定氨基酸残基的替换可显著改变光吸收波长，阐明了视蛋白光谱调谐的结构基础。
4. **"Crystal structure of rhodopsin: implications for vision and beyond"**
- **作者**: Standfuss, J., et al.
- **摘要**: 利用重组技术表达并解析了视蛋白的晶体结构(含OPN1MW同源模型)，揭示了其跨膜螺旋构象与光信号转导机制的关联。
这些文献涵盖了OPN1MW重组蛋白的表达、功能分析及结构研究，为理解其生物学作用提供了关键见解。
**Background of OPN1MW Recombinant Protein**
OPN1MW (opsin 1. medium-wave-sensitive) is a light-sensitive photoreceptor protein encoded by the *OPN1MW* gene, which is critical for color vision in humans. It is expressed in cone cells of the retina and specifically detects medium-wavelength (green) light. Structurally, OPN1MW belongs to the G protein-coupled receptor (GPCR) family, characterized by seven transmembrane domains. Upon binding to 11-*cis*-retinal, it forms a visual pigment that initiates phototransduction upon light activation, triggering a cascade of signals that enable color perception.
The *OPN1MW* gene is located on the X chromosome, making mutations in this gene a common cause of red-green color vision deficiencies, predominantly affecting males. Recombinant OPN1MW protein is generated through heterologous expression systems (e.g., mammalian, insect, or bacterial cells) for structural and functional studies. This engineered protein allows researchers to investigate the molecular mechanisms of phototransduction, ligand-binding dynamics, and genetic mutations linked to color blindness.
In recent years, OPN1MW recombinant protein has become pivotal in developing gene therapies for color vision disorders. For instance, studies using animal models have demonstrated the potential of viral vector-mediated *OPN1MW* gene delivery to restore color discrimination. Additionally, it serves as a tool in optogenetics and biosensor design, leveraging its light-sensing properties. Research also explores its role in understanding retinal degenerative diseases and improving artificial vision technologies.
Overall, OPN1MW recombinant protein bridges basic science and therapeutic innovation, offering insights into visual physiology and advancing treatments for vision-related genetic disorders.
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