| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GPR15 |
| Uniprot No | P49685 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-360aa |
| 氨基酸序列 | MDPEETSVYLDYYYATSPNSDIRETHSHVPYTSVFLPVFYTAVFLTGVLGNLVLMGALHFKPGSRRLIDIFIINLAASDFIFLVTLPLWVDKEASLGLWRTGSFLCKGSSYMISVNMHCSVLLLTCMSVDRYLAIVWPVVSRKFRRTDCAYVVCASIWFISCLLGLPTLLSRELTLIDDKPYCAEKKATPIKLIWSLVALIFTFFVPLLSIVTCYCCIARKLCAHYQQSGKHNKKLKKSIKIIFIVVAAFLVSWLPFNTFKFLAIVSGLRQEHYLPSAILQLGMEVSGPLAFANSCVNPFIYYIFDSYIRRAIVHCLCPCLKNYDFGSSTETSDSHLTKALSTFIHAEDFARRRKRSVSL |
| 预测分子量 | 43.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GPR15重组蛋白的3篇假设参考文献示例(基于领域内常见研究方向推测，实际文献需通过数据库验证)：
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1. **文献名称**: "Expression and Functional Characterization of Recombinant GPR15 in HEK293 Cells"
**作者**: Smith A, et al.
**摘要**: 本研究报道了在HEK293细胞中重组表达GPR15蛋白的方法，通过荧光标记和cAMP信号通路分析，证实其与β-arrestin的相互作用及配体依赖性激活特性，为研究GPR15在肠道免疫中的作用提供工具。
2. **文献名称**: "Structural Insights into GPR15 via Recombinant Protein Crystallography"
**作者**: Zhang L, et al.
**摘要**: 利用大肠杆菌表达系统纯化GPR15重组蛋白，并通过X射线晶体学解析其三维结构，揭示了该受体胞外域的独特构象及潜在配体结合位点，为靶向药物设计奠定基础。
3. **文献名称**: "GPR15 Recombinant Protein Binding Assay Identifies Novel Chemokine Interactions"
**作者**: Lee J, et al.
**摘要**: 开发基于表面等离子共振(SPR)的重组GPR15蛋白体外结合实验，发现其与特定趋化因子的高亲和力结合，提示其在T细胞迁移中的调控机制。
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**注意**：以上为模拟参考文献，实际文献需通过PubMed、Google Scholar等平台以关键词“GPR15 recombinant protein”检索。建议结合具体研究需求筛选近年高影响力论文。
GPR15 (G protein-coupled receptor 15) is an orphan receptor belonging to the class A G protein-coupled receptor (GPCR) family. First identified in the 1990s, it is encoded by the GPR15 gene located on human chromosome 3. Structurally, it features a characteristic seven-transmembrane domain and an N-terminal extracellular domain with potential glycosylation sites. GPR15 gained attention for its role in immune regulation, particularly in T-cell homing to mucosal tissues like the colon and skin. Studies show it facilitates lymphocyte recruitment during inflammation, contributing to gut homeostasis and inflammatory bowel disease (IBD) pathogenesis.
Notably, GPR15 serves as a co-receptor for specific HIV/SIV strains, influencing viral entry into host cells. Its expression in intestinal epithelial and immune cells underscores its dual role in immunity and viral infection. However, its endogenous ligand remained elusive until recent studies suggested C10orf99 (also named GPR15L) as a potential candidate, activating GPR15-mediated signaling pathways.
Recombinant GPR15 protein, produced using mammalian or insect expression systems, retains structural and functional integrity for experimental applications. It is often engineered with tags (e.g., His-tag, Fc-fusion) to aid purification via affinity chromatography. Researchers use it to study receptor-ligand interactions, signaling mechanisms (e.g., β-arrestin recruitment, MAPK pathways), and its role in autoimmune diseases or viral entry. In drug discovery, recombinant GPR15 serves as a target for screening therapeutic compounds aimed at modulating inflammation or blocking HIV infection. Ongoing research focuses on clarifying its tissue-specific functions and therapeutic potential in IBD, psoriasis, and HIV-associated pathologies.
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