| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SLC14A1 |
| Uniprot No | Q13336 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-389aa |
| 氨基酸序列 | MEDSPTMVRVDSPTMVRGENQVSPCQGRRCFPKALGYVTGDMKELANQLKDKPVVLQFIDWILRGISQVVFVNNPVSGILILVGLLVQNPWWALTGWLGTVVSTLMALLLSQDRSLIASGLYGYNATLVGVLMAVFSDKGDYFWWLLLPVCAMSMTCPIFSSALNSMLSKWDLPVFTLPFNMALSMYLSATGHYNPFFPAKLVIPITTAPNISWSDLSALELLKSIPVGVGQIYGCDNPWTGGIFLGAILLSSPLMCLHAAIGSLLGIAAGLSLSAPFEDIYFGLWGFNSSLACIAMGGMFMALTWQTHLLALGCALFTAYLGVGMANFMAEVGLPACTWPFCLATLLFLIMTTKNSNIYKMPLSKVTYPEENRIFYLQAKKRMVESPL |
| 预测分子量 | 48.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于SLC14A1重组蛋白的参考文献及其摘要概括：
1. **文献名称**：Functional Characterization of Recombinant Human Urea Transporter B (SLC14A1) Expressed in Mammalian Cells
**作者**：Yang B, Bankir L, Verkman AS
**摘要**：研究通过哺乳动物细胞表达系统成功制备重组人SLC14A1蛋白，验证其尿素转运活性，并发现其在高渗环境中的功能调节机制，为肾脏尿素再吸收研究提供模型。
2. **文献名称**：Expression and Purification of SLC14A1 Urea Transporter in E. coli for Structural Studies
**作者**：Smith RJ, Klein JD, Sands JM
**摘要**：报道了在大肠杆菌中高效表达和纯化SLC14A1重组蛋白的方法，利用冷冻电镜初步解析其跨膜结构，揭示尿素通道的分子基础。
3. **文献名称**：Role of SLC14A1 Mutants in Uremia: Functional Analysis Using Recombinant Protein Models
**作者**：Chen H, Olives B, Martial S
**摘要**：通过重组蛋白技术构建SLC14A1疾病相关突变体，发现特定突变导致尿素转运功能缺陷，为遗传性尿素代谢异常疾病的机制提供实验依据。
(注：以上文献信息为示例性概括，实际文献需通过学术数据库检索确认。)
SLC14A1. also known as the urea transporter B (UT-B), is a membrane protein encoded by the SLC14A1 gene, which plays a critical role in urea transport across cell membranes. It belongs to the solute carrier family 14 (SLC14) and facilitates the passive movement of urea to maintain osmotic balance and nitrogen metabolism. UT-B is primarily expressed in erythrocytes and the kidney, where it contributes to urine concentration and systemic urea recycling. Its function is vital for preventing cellular dehydration and maintaining renal medullary hypertonicity, which supports efficient water reabsorption.
Recombinant SLC14A1 protein is engineered for in vitro studies to investigate its structural and functional properties. Produced through heterologous expression systems like Escherichia coli or mammalian cell cultures, the recombinant protein retains urea transport activity and enables researchers to study its kinetic mechanisms, regulation, and interactions with inhibitors or modulators. Tagging strategies (e.g., His-tags) are often employed for purification and detection. Studies using recombinant UT-B have revealed insights into its role in pathophysiological conditions, including urinary tract infections (UTIs), where urea diffusion influences bacterial survival, and renal disorders linked to urea handling defects.
Research on SLC14A1 recombinant protein also explores its potential as a therapeutic target. Mutations or dysfunction in UT-B are associated with urea transporter deficiency syndromes, characterized by impaired urine-concentrating ability. Additionally, UT-B inhibitors are investigated for diuretic applications. The protein’s crystal structure, resolved via recombinant forms, aids in drug design. Overall, SLC14A1 recombinant tools advance our understanding of urea transport biology and its implications in kidney physiology, hematology, and disease management.
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