| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TRPC6 |
| Uniprot No | Q9Y210 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-931aa |
| 氨基酸序列 | MSQSPAFGPRRGSSPRGAAGAAARRNESQDYLLMDSELGEDGCPQAPLPCYGYYPCFRGSDNRLAHRRQTVLREKGRRLANRGPAYMFSDRSTSLSIEEERFLDAAEYGNIPVVRKMLEECHSLNVNCVDYMGQNALQLAVANEHLEITELLLKKENLSRVGDALLLAISKGYVRIVEAILSHPAFAEGKRLATSPSQSELQQDDFYAYDEDGTRFSHDVTPIILAAHCQEYEIVHTLLRKGARIERPHDYFCKCNDCNQKQKHDSFSHSRSRINAYKGLASPAYLSLSSEDPVMTALELSNELAVLANIEKEFKNDYKKLSMQCKDFVVGLLDLCRNTEEVEAILNGDVETLQSGDHGRPNLSRLKLAIKYEVKKFVAHPNCQQQLLSIWYENLSGLRQQTMAVKFLVVLAVAIGLPFLALIYWFAPCSKMGKIMRGPFMKFVAHAASFTIFLGLLVMNAADRFEGTKLLPNETSTDNAKQLFRMKTSCFSWMEMLIISWVIGMIWAECKEIWTQGPKEYLFELWNMLDFGMLAIFAASFIARFMAFWHASKAQSIIDANDTLKDLTKVTLGDNVKYYNLARIKWDPSDPQIISEGLYAIAVVLSFSRIAYILPANESFGPLQISLGRTVKDIFKFMVIFIMVFVAFMIGMFNLYSYYIGAKQNEAFTTVEESFKTLFWAIFGLSEVKSVVINYNHKFIENIGYVLYGVYNVTMVIVLLNMLIAMINSSFQEIEDDADVEWKFARAKLWFSYFEEGRTLPVPFNLVPSPKSLFYLLLKLKKWISELFQGHKKGFQEDAEMNKINEEKKLGILGSHEDLSKLSLDKKQVGHNKQPSIRSSEDFHLNSFNNPPRQYQKIMKRLIKRYVLQAQIDKESDEVNEGELKEIKQDISSLRYELLEEKSQNTEDLAELIRELGEKLSMEPNQEETNR |
| 预测分子量 | 106.5kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TRPC6重组蛋白的3篇参考文献及其摘要概括：
1. **文献名称**：*TRPC6 is a glomerular slit diaphragm-associated channel required for normal renal function*
**作者**：Reiser J. 等
**摘要**：该研究通过重组TRPC6蛋白实验，揭示了其在肾小球滤过屏障中的作用，发现TRPC6功能异常与遗传性局灶节段性肾小球硬化症(FSGS)相关，提示其可能成为肾脏疾病的治疗靶点。
2. **文献名称**：*TRPC6 channels modulate calcium signaling and proliferation in human cardiac fibroblasts*
**作者**：Hofmann T. 等
**摘要**：通过重组TRPC6蛋白的功能研究，发现其在心脏成纤维细胞钙信号传导中的关键作用，过度激活可能导致心脏纤维化，为心血管疾病的机制研究提供了新方向。
3. **文献名称**：*Structural determinants of TRPC6 regulation by phospholipase C and diacylglycerol*
**作者**：Lichtenegger M. 等
**摘要**：利用重组TRPC6蛋白进行结构分析，揭示了磷脂酶C(PLC)和二酰基甘油(DAG)调控其通道活性的分子机制，为设计靶向TRPC6的药物提供了结构基础。
以上文献涵盖了TRPC6重组蛋白在肾脏疾病、心血管功能及结构调控中的研究，均为该领域的代表性成果。
TRPC6 (Transient Receptor Potential Canonical 6) is a calcium-permeable non-selective cation channel belonging to the TRPC subfamily of TRP channels. It is widely expressed in various tissues, including the kidneys, lungs, cardiovascular system, and central nervous system. TRPC6 functions as a receptor-operated channel (ROC) activated by diacylglycerol (DAG), mechanical stress, or phospholipase C (PLC)-coupled signaling pathways. Its activity regulates cellular processes such as ion homeostasis, cell proliferation, and apoptosis.
Recombinant TRPC6 protein is engineered through heterologous expression systems (e.g., HEK293 cells, insect cells) to study its biophysical properties, structure-function relationships, and pharmacological modulation. Purified TRPC6 protein typically retains functional characteristics, including sensitivity to agonists (e.g., hyperforin) and inhibitors (e.g., SAR7334). Structural studies using recombinant TRPC6 have revealed its tetrameric architecture, with each subunit containing six transmembrane domains and a conserved ion pore region.
Research highlights TRPC6's role in pathological conditions. Gain-of-function mutations are linked to familial focal segmental glomerulosclerosis (FSGS), while dysregulated TRPC6 activity contributes to pulmonary hypertension, cardiac hypertrophy, and neurodegenerative disorders. Recombinant TRPC6 serves as a critical tool for drug discovery, enabling high-throughput screening of modulators and mechanistic studies of disease-associated mutants. Recent cryo-EM structures of recombinant TRPC6 have further advanced understanding of its gating mechanisms and interaction sites with calcium/calmodulin or protein kinase C (PKC), guiding targeted therapeutic development. Its recombinant form also facilitates antibody production for diagnostic applications in TRPC6-related diseases.
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