| 纯度 | > 90 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | AKR1C4 |
| Uniprot No | P17516 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-343aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMDPKYQRVELNDGHFMPVLGFGTYAPPEVP RNRAVEVTKLAIEAGFRHIDSAYLYNNEEQVGLAIRSKIADGSVKREDIF YTSKLWCTFFQPQMVQPALESSLKKLQLDYVDLYLLHFPMALKPGETPLP KDENGKVIFDTVDLSATWEVMEKCKDAGLAKSIGVSNFNCRQLEMILNKP GLKYKPVCNQVECHPYLNQSKLLDFCKSKDIVLVAHSALGTQRHKLWVDP NSPVLLEDPVLCALAKKHKRTPALIALRYQLQRGVVVLAKSYNEQRIREN IQVFEFQLTSEDMKVLDGLNRNYRYVVMDFLMDHPDYPFSDEY |
| 预测分子量 | 39 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于AKR1C4重组蛋白的3篇代表性文献摘要(注：文献为模拟示例，具体内容请参考实际数据库)：
---
1. **文献名称**：*Expression and characterization of recombinant human AKR1C4 in Escherichia coli*
**作者**：Smith J, et al.
**摘要**：研究报道了人源AKR1C4蛋白在大肠杆菌中的重组表达及纯化方法，并分析了其对类固醇底物(如孕酮、雄烯二酮)的催化活性，证实其在类固醇激素代谢中的关键作用。
2. **文献名称**：*Structural basis for substrate specificity of AKR1C4 in steroid hormone metabolism*
**作者**：Li X, Wang Y.
**摘要**：通过X射线晶体学解析了AKR1C4重组蛋白的三维结构，揭示了其活性口袋与类固醇底物的结合模式，阐明了其催化还原反应的结构基础。
3. **文献名称**：*AKR1C4 overexpression promotes drug resistance in hepatocellular carcinoma via prostaglandin metabolism*
**作者**：Chen R, et al.
**摘要**：研究发现肝癌细胞中AKR1C4重组蛋白的异常高表达可通过调控前列腺素代谢通路增强化疗耐药性，提示其作为肿瘤治疗靶点的潜在价值。
---
如需具体文献，建议通过PubMed或Web of Science以“AKR1C4 recombinant protein”为关键词检索。
**Background of AKR1C4 Recombinant Protein**
AKR1C4 (aldo-keto reductase family 1 member C4), also known as hydroxysteroid dehydrogenase or chlordecone reductase, is a member of the aldo-keto reductase superfamily. This enzyme plays a critical role in steroid hormone metabolism, particularly in the reduction of ketosteroids, prostaglandins, and other carbonyl-containing substrates. It is involved in the biosynthesis and inactivation of steroid hormones, such as converting active androgens into less potent forms and regulating estrogen metabolism. AKR1C4 is predominantly expressed in the liver, though it is also found in steroidogenic tissues, highlighting its importance in systemic hormonal balance.
Recombinant AKR1C4 protein is produced using genetic engineering techniques, where the *AKR1C4* gene is cloned into expression vectors (e.g., bacterial, insect, or mammalian systems) to enable large-scale protein production. This approach ensures high purity and consistency, making it valuable for biochemical studies. Researchers utilize recombinant AKR1C4 to investigate its enzymatic kinetics, substrate specificity, and interactions with inhibitors or drugs. Its role in hormone-dependent diseases, such as cancers (e.g., prostate, breast) and metabolic disorders, has spurred interest in targeting AKR1C4 for therapeutic development.
Studies also explore its involvement in detoxification pathways, particularly in metabolizing xenobiotics and lipid peroxidation products. Structural analyses of recombinant AKR1C4 have provided insights into its catalytic mechanism and active-site architecture, aiding rational drug design. Challenges include understanding tissue-specific regulation and isoform redundancy within the AKR1C subfamily. Overall, AKR1C4 recombinant protein serves as a vital tool for unraveling its physiological roles and potential clinical applications.
×
