| 纯度 | > 85 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | ALDH6A1 |
| Uniprot No | P51647 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 34-535aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSSSSVPTV KLFIGGKFVE SKSDKWIDIH NPATNEVIGR VPQATKAEMD AAIASCKRAF PAWADTSVLS RQQVLLRYQQ LIKENLKEIA KLITLEQGKT LADAEGDVFR GLQVVEHACS VTSLMMGETM PSITKDMDLY SYRLPLGVCA GIAPFNFPAM IPLWMFPMAM VCGNTFLMKP SERVPGATML LAKLLQDSGA PDGTLNIIHG QHEAVNFICD HPDIKAISFV GSNKAGEYIF ERGSRHGKRV QANMGAKNHG VVMPDANKEN TLNQLVGAAF GAAGQRCMAL STAVLVGEAK KWLPELVEHA KNLRVNAGDQ PGADLGPLIT PQAKERVCNL IDSGTKEGAS ILLDGRKIKV KGYENGNFVG PTIISNVKPN MTCYKEEIFG PVLVVLETET LDEAIQIVNN NPYGNGTAIF TTNGATARKY AHLVDVGQVG VNVPIPVPLP MFSFTGSRSS FRGDTNFYGK QGIQFYTQLK TITSQWKEED ATLSSPAVVM PTMGR |
| 预测分子量 | 57 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ALDH6A1重组蛋白的3篇参考文献示例(文献信息为模拟内容，仅供参考)：
1. **文献名称**：*Functional characterization of recombinant human ALDH6A1: Role in valine and pyrimidine metabolism*
**作者**：Smith J, et al.
**摘要**：研究通过在大肠杆菌中表达重组人ALDH6A1蛋白，证实其催化丙酸代谢中间体(如甲基丙二酸半醛)的脱氢酶活性，并发现其参与调节线粒体内支链氨基酸及嘧啶代谢通路。
2. **文献名称**：*Crystal structure and substrate specificity of ALDH6A1: Insights into its metabolic implications*
**作者**：Chen L, et al.
**摘要**：解析了重组ALDH6A1蛋白的晶体结构，揭示了其底物结合口袋的关键氨基酸残基，并通过酶动力学实验证明其对丙酰辅酶A衍生物的高亲和力，提示其在先天性代谢缺陷中的潜在作用。
3. **文献名称**：*ALDH6A1 deficiency alters mitochondrial acetyl-CoA homeostasis and promotes cellular oxidative stress*
**作者**：Wang Y, et al.
**摘要**：利用重组ALDH6A1蛋白进行体外功能研究，发现其缺失导致线粒体乙酰辅酶A失衡，引发活性氧(ROS)累积，为ALDH6A1突变相关代谢疾病的分子机制提供了依据。
(注：以上文献为示例，实际文献需通过PubMed、Google Scholar等平台以“ALDH6A1 recombinant protein”为关键词检索。)
ALDH6A1 (Aldehyde Dehydrogenase 6 Family Member A1) is a mitochondrial enzyme encoded by the ALDH6A1 gene, playing a critical role in amino acid and fatty acid metabolism. It belongs to the aldehyde dehydrogenase (ALDH) superfamily, which catalyzes the oxidation of aldehydes to carboxylic acids. Specifically, ALDH6A1 functions as a methylmalonate semialdehyde dehydrogenase, converting methylmalonate semialdehyde (MMSA) to acetyl-CoA during the catabolism of valine, thymine, and cholesterol. This reaction links the breakdown of branched-chain amino acids and odd-chain fatty acids to the tricarboxylic acid (TCA) cycle, underscoring its importance in energy production and metabolic homeostasis.
Recombinant ALDH6A1 protein is engineered for in vitro studies to elucidate its structural, enzymatic, and regulatory properties. Produced using expression systems like E. coli or mammalian cells, the purified protein retains enzymatic activity, enabling researchers to investigate substrate specificity, cofactor requirements (e.g., NAD⁺ dependence), and kinetic parameters. Its recombinant form is instrumental in studying genetic mutations associated with metabolic disorders. For instance, ALDH6A1 deficiency is linked to methylmalonic aciduria, a rare autosomal recessive condition characterized by elevated methylmalonic acid levels, developmental delays, and neurological impairments.
Research on recombinant ALDH6A1 also explores its potential role in cancer and metabolic syndromes, as altered ALDH activity is implicated in oxidative stress and disease progression. Structural studies using recombinant protein have revealed insights into its homotetrameric organization and substrate-binding domains, aiding drug discovery efforts. Overall, ALDH6A1 recombinant protein serves as a vital tool for decoding metabolic pathways, disease mechanisms, and therapeutic targeting.
×
