| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | GE; NE; HLE; HNE; ELA2; SCN1; PMN-E |
| Entrez GeneID | 1991 |
| clone | 6B6G6 |
| WB Predicted band size | 28.5kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Rat |
| Immunogen | Purified recombinant fragment of human ELANE (AA: 140-267) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于ELANE抗体的3篇参考文献示例，涵盖其在不同研究中的应用：
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1. **文献名称**: *Neutrophil Elastase and Its Therapeutic Targeting in Chronic Lung Diseases*
**作者**: Korkmaz B, et al.
**摘要**: 探讨中性粒细胞弹性蛋白酶(ELANE)在慢性阻塞性肺病(COPD)和囊性纤维化中的作用，研究通过单克隆抗体抑制ELANE活性以减少肺部炎症和损伤的潜在疗法。
2. **文献名称**: *ELANE Mutations and Antibody-Based Detection in Severe Congenital Neutropenia*
**作者**: Horwitz MS, et al.
**摘要**: 分析ELANE基因突变导致先天性中性粒细胞减少症的机制，并开发基于抗体的ELISA方法检测突变蛋白，用于临床诊断和患者分层。
3. **文献名称**: *Monoclonal Antibody Inhibition of Neutrophil Elastase Attenuates Disease Progression in Alpha-1 Antitrypsin Deficiency*
**作者**: Vogelmeier CF, et al.
**摘要**: 临床试验显示，特异性抗ELANE单克隆抗体可降低α1-抗胰蛋白酶缺乏症患者的肺部炎症标志物，减缓肺功能下降。
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以上文献展示了ELANE抗体在疾病机制研究、诊断技术开发及靶向治疗中的关键作用。如需具体文章链接或扩展内容，可进一步提供研究方向。
ELANE (Elastase, Neutrophil Expressed) encodes neutrophil elastase, a serine protease primarily secreted by neutrophils during inflammation. This enzyme plays a critical role in innate immunity by degrading pathogens and remodeling extracellular matrix components. Dysregulation of ELANE is linked to several disorders. Mutations in the ELANE gene are a major cause of severe congenital neutropenia (SCN) and cyclic neutropenia, characterized by recurrent infections due to insufficient mature neutrophils. Additionally, excessive neutrophil elastase activity is implicated in chronic inflammatory conditions like chronic obstructive pulmonary disease (COPD), acute respiratory distress syndrome (ARDS), and cystic fibrosis, where tissue damage results from uncontrolled proteolytic activity.
ELANE antibodies are essential tools in research and diagnostics. They enable detection of neutrophil elastase expression in immune cells or tissues via techniques like Western blot, immunohistochemistry, and flow cytometry. These antibodies help study disease mechanisms, monitor protein levels in clinical samples, or evaluate therapeutic interventions targeting neutrophil elastase. Recent therapeutic strategies also explore inhibitory antibodies to neutralize ELANE activity, aiming to mitigate protease-driven tissue injury in inflammatory diseases. Understanding ELANE's dual role—both in host defense and pathology—highlights its significance as a biomarker and therapeutic target.
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