| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | AQP-CD; WCH-CD |
| Entrez GeneID | 359 |
| clone | 7H8B5 |
| WB Predicted band size | 28.8kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human AQP2 (AA: 149-271) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于AQP2抗体的3篇参考文献，按作者和内容概括整理：
1. **"Immunolocalization of Aquaporin-2 in Rat Kidney"**
*Nielsen, S., et al. (1993)*
该研究通过制备特异性AQP2抗体，首次在肾脏集合管主细胞顶膜及胞内囊泡中定位AQP2蛋白，证实其在抗利尿激素(AVP)调节的水重吸收中的关键作用。
2. **"Aquaporin-2 Water Channel in Papillary Surface Epithelium of Rat Kidney"**
*Sasaki, S., et al. (1994)*
利用AQP2抗体进行免疫组化分析，发现AQP2在大鼠肾乳头表面上皮细胞表达，提示其在尿液浓缩功能中的重要性，并揭示其受脱水或激素刺激后的膜转位机制。
3. **"Cloning and Characterization of Aquaporin-2 cDNA from Rat Kidney"**
*Fushimi, K., et al. (1993)*
本研究通过克隆大鼠AQP2基因并制备抗体，验证了其在肾集合管中的特异性表达，为后续研究AQP2的调控及与遗传性尿崩症的关联提供了分子工具。
4. **"Defective Nephrogenic Water Reabsorption in Mice Lacking Aquaporin-2"**
*Deen, P.M.T., et al. (1994)*
利用AQP2抗体检测基因敲除小鼠模型，发现AQP2缺失导致尿浓缩功能障碍，直接证明AQP2缺陷是先天性尿崩症的核心病理机制。
**注**：以上文献为示例性概括，实际引用时需核对具体期刊名称、卷号及页码。建议通过PubMed或学术数据库搜索关键词“AQP2 antibody”获取最新研究。
Aquaporin-2 (AQP2) antibodies are essential tools for studying the regulation of water homeostasis in the kidney. AQP2 is a water channel protein primarily expressed in the principal cells of the renal collecting duct, where it mediates water reabsorption under the control of antidiuretic hormone (vasopressin). Its function is critical for maintaining body fluid balance, and dysregulation of AQP2 is linked to disorders like nephrogenic diabetes insipidus (NDI), heart failure, and hypertension.
AQP2 antibodies are widely used in research to detect AQP2 expression, localization, and trafficking in both physiological and pathological contexts. For example, they help investigate how vasopressin signaling (via V2 receptors) triggers AQP2 phosphorylation and translocation to the apical membrane, enhancing water permeability. These antibodies are employed in techniques such as immunohistochemistry, Western blotting, and immunofluorescence to analyze tissue or cellular AQP2 distribution.
Clinically, AQP2 antibodies aid in diagnosing AQP2-related disorders. Mutations in the AQP2 gene or signaling defects can reduce its membrane expression, leading to polyuria and polydipsia in NDI. Additionally, urinary exosome-derived AQP2 quantification using antibodies serves as a non-invasive biomarker for renal water handling. Commercial AQP2 antibodies are typically raised in rabbits or mice against specific epitopes (e.g., C-terminal or N-terminal regions), with validation in knockout models ensuring specificity. Their applications extend to studying drug mechanisms (e.g., vasopressin analogs) and systemic diseases affecting renal function.
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