| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SNRPA |
| Uniprot No | P09012 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-282aa |
| 氨基酸序列 | AVPETRPNHTIYINNLNEKIKKDELKKSLYAIFSQFGQILDILVSRSLKMRGQAFVIFKEVSSATNALRSMQGFPFYDKPMRIQYAKTDSDIIAKMKGTFVERDRKREKRKPKSQETPATKKAVQGGGATPVVGAVQGPVPGMPPMTQAPRIMHHMPGQPPYMPPPGMIPPPGLAPGQIPPGAMPPQQLMPGQMPPAQPLSENPPNHILFLTNLPEETNELMLSMLFNQFPGFKEVRLVPGRHDIAFVEFDNEVQAGAARDALQGFKITQNNAMKISFAKK |
| 预测分子量 | 38.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SNRPA(U1 snRNP核心蛋白)重组蛋白研究的示例文献摘要，结合真实研究方向和作者领域整理而成。请注意，这些为示例性描述，具体文献需通过学术数据库进一步验证：
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1. **文献名称**：*"Recombinant Production and Structural Analysis of Human SNRPA in Complex with snRNA"*
**作者**：Nagai, K., et al.
**摘要**：本研究利用大肠杆菌表达系统成功表达了重组人源SNRPA蛋白，并通过亲和层析纯化。结合X射线晶体学，揭示了SNRPA与U1 snRNA的相互作用机制，为剪接体组装提供了结构基础。
2. **文献名称**：*"Autoantigenicity of Recombinant SNRPA in Systemic Lupus Erythematosus Diagnosis"*
**作者**：Hoch, S.O., et al.
**摘要**：通过重组SNRPA蛋白检测系统性红斑狼疮(SLE)患者血清中的自身抗体，证实其作为高特异性抗原的潜力，为临床诊断工具开发提供了依据。
3. **文献名称**：*"Functional Reconstitution of the U1 snRNP Complex Using Recombinant SNRPA and Sm Proteins"*
**作者**：Lührmann, C., et al.
**摘要**：通过重组SNRPA与Sm家族蛋白在体外重构功能性U1 snRNP复合物，阐明了其在pre-mRNA剪接中的协同作用机制。
4. **文献名称**：*"Optimization of SNRPA Expression in Mammalian Cells for Spliceosome Assembly Studies"*
**作者**：Jurica, M.S., et al.
**摘要**：采用哺乳动物表达系统优化重组SNRPA的产量，结合生化实验验证其在剪接体动态组装中的关键功能。
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**建议**：如需真实文献，可在PubMed或Google Scholar中搜索关键词“SNRPA recombinant expression”、“U1 snRNP assembly”或结合具体研究方向筛选。
SNRPA (Small Nuclear Ribonucleoprotein Polypeptide A), also known as U1-A, is a key protein component of the U1 small nuclear ribonucleoprotein (U1 snRNP) complex, which plays a central role in pre-mRNA splicing. This protein contains an RNA recognition motif (RRM) domain that binds specifically to the stem-loop II region of U1 snRNA, stabilizing the U1 snRNP structure and facilitating its assembly into the spliceosome. SNRPA is essential for the recognition of 5' splice sites during the early stages of spliceosome assembly, making it critical for accurate removal of introns and proper gene expression.
Recombinant SNRPA protein is typically produced using expression systems like *E. coli* or mammalian cell cultures, enabling studies of its molecular interactions and structural properties. Researchers utilize purified SNRPA to investigate splicing mechanisms, protein-RNA binding dynamics, and spliceosome assembly pathways. Its recombinant form has been instrumental in structural studies, including X-ray crystallography and cryo-EM, revealing how RRM domains engage with RNA targets.
Dysregulation of SNRPA or U1 snRNP function has been linked to diseases such as spinal muscular atrophy, neurodegenerative disorders, and cancers. Abnormal splicing events caused by SNRPA mutations or altered expression may contribute to disease pathogenesis, highlighting its potential as a therapeutic target. Recombinant SNRPA also serves as a tool for screening small molecules that modulate splicing activity or repair aberrant splicing in genetic disorders.
Despite its well-characterized role, questions remain about SNRPA's post-translational modifications, isoform-specific functions, and interactions with auxiliary splicing factors. Ongoing research aims to unravel its context-dependent roles in tissue-specific splicing and disease models.
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