| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Rps6ka4 |
| Uniprot No | O75676 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-772aa |
| 氨基酸序列 | MGDEDDDESC AVELRITEAN LTGHEEKVSV ENFELLKVLG TGAYGKVFLV RKAGGHDAGK LYAMKVLRKA ALVQRAKTQE HTRTERSVLE LVRQAPFLVT LHYAFQTDAK LHLILDYVSG GEMFTHLYQR QYFKEAEVRV YGGEIVLALE HLHKLGIIYR DLKLENVLLD SEGHIVLTDF GLSKEFLTEE KERTFSFCGT IEYMAPEIIR SKTGHGKAVD WWSLGILLFE LLTGASPFTL EGERNTQAEV SRRILKCSPP FPPRIGPVAQ DLLQRLLCKD PKKRLGAGPQ GAQEVRNHPF FQGLDWVALA ARKIPAPFRP QIRSELDVGN FAEEFTRLEP VYSPPGSPPP GDPRIFQGYS FVAPSILFDH NNAVMTDGLE APGAGDRPGR AAVARSAMMQ DSPFFQQYEL DLREPALGQG SFSVCRRCRQ RQSGQEFAVK ILSRRLEANT QREVAALRLC QSHPNVVNLH EVHHDQLHTY LVLELLRGGE LLEHIRKKRH FSESEASQIL RSLVSAVSFM HEEAGVVHRD LKPENILYAD DTPGAPVKII DFGFARLRPQ SPGVPMQTPC FTLQYAAPEL LAQQGYDESC DLWSLGVILY MMLSGQVPFQ GASGQGGQSQ AAEIMCKIRE GRFSLDGEAW QGVSEEAKEL VRGLLTVDPA KRLKLEGLRG SSWLQDGSAR SSPPLRTPDV LESSGPAVRS GLNATFMAFN RGKREGFFLK SVENAPLAKR RKQKLRSATA SRRGSPAPAN PGRAPVASKG APRRANGPLP PS |
| 预测分子量 | 85.6kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于Rps6ka4(RSK4)重组蛋白的示例参考文献(注：以下为虚拟示例，实际文献需通过学术数据库检索确认)：
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1. **文献名称**: *"Recombinant Expression and Functional Characterization of RPS6KA4 in MAPK Signaling"*
**作者**: Zhang L, et al.
**摘要**: 本研究报道了在大肠杆菌系统中高效表达并纯化Rps6ka4重组蛋白的流程，通过体外激酶实验证实其磷酸化下游底物CREB的能力，并揭示其在MAPK/ERK信号通路中的调控作用。
2. **文献名称**: *"Structural Insights into RSK4 Kinase Domain via Recombinant Protein Crystallography"*
**作者**: Müller S, et al.
**摘要**: 利用昆虫细胞表达系统获得Rps6ka4激酶结构域的重组蛋白，解析其晶体结构至2.1Å分辨率，揭示了ATP结合口袋的独特构象，为靶向RSK4的抑制剂设计提供结构基础。
3. **文献名称**: *"RSK4 Recombinant Protein Suppresses Tumor Growth in Triple-Negative Breast Cancer Models"*
**作者**: Chen H, et al.
**摘要**: 通过哺乳动物细胞表达系统制备功能性Rps6ka4重组蛋白，体外实验表明其过表达可抑制TNBC细胞增殖，并在小鼠移植瘤模型中观察到肿瘤体积显著减小，提示其潜在抑癌功能。
4. **文献名称**: *"Development of a High-Throughput Assay for RSK4 Kinase Activity Using Recombinant Protein"*
**作者**: Gupta R, et al.
**摘要**: 建立基于荧光共振能量转移(FRET)的RSK4激酶活性检测方法，利用纯化的重组Rps6ka4蛋白筛选小分子库，鉴定出多个新型选择性抑制剂，推动RSK4相关疾病药物开发。
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建议通过PubMed或Web of Science搜索关键词“RPS6KA4 recombinant”、“RSK4 protein expression”获取真实文献。
**Background of Rps6ka4 Recombinant Protein**
Rps6ka4 (Ribosomal Protein S6 Kinase A4), also known as MSK2 or MAPKAPK1B, is a serine/threonine kinase belonging to the RSK (p90 ribosomal S6 kinase) family. It plays a critical role in the mitogen-activated protein kinase (MAPK) signaling pathway, which regulates diverse cellular processes, including proliferation, survival, differentiation, and stress responses. Rps6ka4 is activated by upstream kinases such as ERK1/2 or p38 MAPK through phosphorylation, enabling it to phosphorylate downstream targets, including transcription factors (e.g., CREB, NF-κB) and histone H3. thereby modulating gene expression and chromatin remodeling.
The recombinant Rps6ka4 protein is engineered in vitro using expression systems (e.g., *E. coli* or mammalian cells) to ensure high purity and bioactivity. Its structure typically retains functional domains, such as the N-terminal kinase domain and C-terminal regulatory region, essential for substrate recognition and catalytic activity. Recombinant Rps6ka4 is widely used to study MAPK/RSK signaling mechanisms, screen kinase inhibitors for therapeutic development (e.g., targeting cancer or inflammatory diseases), and analyze protein-protein interactions.
Dysregulation of Rps6ka4 has been linked to pathologies like cancer, neurodegeneration, and immune disorders, underscoring its biomedical relevance. Research utilizing recombinant Rps6ka4 helps elucidate its role in disease pathways and supports drug discovery efforts. Quality validation often includes SDS-PAGE, Western blotting, and kinase activity assays to confirm structural integrity and functionality.
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