| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | pepT |
| Uniprot No | Q8XPD8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-406aa |
| 氨基酸序列 | MKKVHERFLEYVKVDTKSDETTRVTPSTKGQLELGKMLAEELKEIGVDEVRISEEGYVYACLKSNCNKDIPKIGFISHMDTAPDMSGKNVNPKIVENYDGKDIELGNGYTLSPSFSPELPMYKGQTLITTDGTTLLGADDKAGIAEIVTAIEYLINHPEIKHGDIKIGFTPDEEIGEGADHFDVEGFGADFAYTLDGGRIGELEYENFNAASAKVEIIGKNVHPGSAKGKMINSILVAHEFVSMLPLDEVPEKTEGYEGFSFLLDIQGEVEKTSLSFIIRDFDKEGFKNRKERFNEIAKELNKKYGEGTVTVTLKDQYMNMKEMIEPRMHIVETAEKAMKQCGIEPIKNPIRGGTDGARLSFMGLPTPNLFTGGENFHGRYEYISINSMEKAVEVILNIIKIYAEK |
| 预测分子量 | 46.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为3条关于pepT重组蛋白的示例参考文献(注：文献内容为虚构示例，仅供格式参考)：
1. **《Heterologous expression and functional characterization of the bacterial peptide transporter PepT in E. coli》**
- 作者：Smith J, et al.
- 摘要：本研究利用大肠杆菌表达系统成功重组表达了PepT蛋白，并验证其肽转运活性。通过体外功能实验证实PepT对二肽类底物的特异性结合能力，为后续药物递送研究提供基础。
2. **《Structural insights into the mechanism of PepT-mediated peptide uptake》**
- 作者：Chen L, et al.
- 摘要：通过X射线晶体学解析了重组PepT蛋白的三维结构，揭示了其跨膜结构域中关键的底物结合位点，并发现pH依赖性构象变化对转运效率的调控作用。
3. **《Engineering recombinant PepT for enhanced stability in therapeutic applications》**
- 作者：Wang Y, et al.
- 摘要：通过定点突变优化PepT重组蛋白的热稳定性及耐酶解能力，证明改造后的蛋白在模拟肠液环境中保持活性，可能作为口服药物载体应用于生物医药领域。
(提示：实际研究中请通过PubMed或Web of Science检索真实文献，关键词可尝试 "PepT recombinant protein" 或结合具体物种名称如 "Lactobacillus PepT")
**Background of pepT Recombinant Protein**
The pepT recombinant protein is a engineered version of the peptide transporter PepT, a member of the proton-dependent oligopeptide transporter (POT) family. PepT proteins are integral membrane proteins that facilitate the uptake of di- and tripeptides across cellular membranes, leveraging proton gradients for active transport. These transporters play critical roles in nutrient absorption, cellular signaling, and immune response modulation. In humans, homologs like PepT1 (SLC15A1) and PepT2 (SLC15A2) are well-studied for their roles in intestinal peptide absorption and renal reabsorption, respectively.
The recombinant pepT protein is typically expressed in heterologous systems, such as *E. coli* or mammalian cell lines, using genetic engineering techniques. Its production enables detailed structural and functional studies, including substrate specificity, transport mechanisms, and interactions with pharmaceuticals. PepT transporters are of particular interest in drug delivery due to their ability to transport peptide-like drugs, such as β-lactam antibiotics or antiviral agents, enhancing oral bioavailability.
Research on recombinant pepT also extends to microbial systems. In pathogens like *Lactococcus* or *Streptococcus*, PepT homologs contribute to nutrient scavenging and virulence, making them potential targets for antimicrobial strategies. Structural studies using recombinant pepT have revealed conserved features, such as a large hydrophilic cavity and transmembrane helices critical for substrate binding and proton coupling.
Overall, pepT recombinant proteins serve as essential tools for deciphering transport physiology, optimizing therapeutic delivery, and exploring microbial pathogenesis, bridging fundamental biology with biotechnological applications.
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