| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HK4 |
| Uniprot No | Q14397 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-625aa |
| 氨基酸序列 | MPGTKRFQHVIETPEPGKWELSGYEAAVPITEKSNPLTQDLDKADAENIVRLLGQCDAEIFQEEGQALSTYQRLYSESILTTMVQVAGKVQEVLKEPDGGLVVLSGGGTSGRMAFLMSVSFNQLMKGLGQKPLYTYLIAGGDRSVVASREGTEDSALHGIEELKKVAAGKKRVIVIGISVGLSAPFVAGQMDCCMNNTAVFLPVLVGFNPVSMARNDPIEDWSSTFRQVAERMQKMQEKQKAFVLNPAIGPEGLSGSSRMKGGSATKILLETLLLAAHKTVDQGIAASQRCLLEILRTFERAHQVTYSQSPKIATLMKSVSTSLEKKGHVYLVGWQTLGIIAIMDGVECIHTFGADFRDVRGFLIGDHSDMFNQKAELTNQGPQFTFSQEDFLTSILPSLTEIDTVVFIFTLDDNLTEVQTIVEQVKEKTNHIQALAHSTVGQTLPIPLKKLFPSIISITWPLLFFEYEGNFIQKFQRELSTKWVLNTVSTGAHVLLGKILQNHMLDLRISNSKLFWRALAMLQRFSGQSKARCIESLLRAIHFPQPLSDDIRAAPISCHVQVAHEKEQVIPIALLSLLFRCSITEAQAHLAAAPSVCEAVRSALAGPGQKRTADPLEILEPDVQ |
| 预测分子量 | 70.7kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于“HK4重组蛋白”的模拟参考文献示例(注：HK4的指代可能存在差异，建议核实具体研究背景或蛋白全称)：
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1. **文献名称**: *Expression and Functional Characterization of Recombinant HK4 Protein in Mammalian Cells*
**作者**: Wang L, et al.
**摘要**: 研究报道了在哺乳动物细胞中成功表达HK4重组蛋白，并验证其磷酸激酶活性，揭示了其在细胞信号转导中的潜在作用。
2. **文献名称**: *HK4 Recombinant Protein Purification and Structural Analysis*
**作者**: Gupta R, Patel S.
**摘要**: 通过亲和层析技术纯化HK4重组蛋白，结合质谱和圆二色谱分析其结构，为靶向药物开发提供结构基础。
3. **文献名称**: *Role of HK4 in Metabolic Regulation: Insights from Recombinant Protein Studies*
**作者**: Kim H, et al.
**摘要**: 利用重组HK4蛋白进行体外实验，证明其参与糖代谢调控，可能与胰岛素抵抗相关疾病的病理机制有关。
4. **文献名称**: *HK4 Recombinant Protein as a Diagnostic Antigen for Autoimmune Diseases*
**作者**: Müller F, et al.
**摘要**: 评估HK4重组蛋白作为自身免疫性疾病血清学标志物的潜力，显示其在患者抗体检测中的高特异性。
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**注意**：上述文献为模拟示例，实际研究中“HK4”可能指代不同蛋白(如己糖激酶变体、特定基因产物或研究代号)。建议通过学术数据库(如PubMed、Web of Science)结合具体研究领域的关键词(如“HK4 protein”“recombinant HK4”“HK4 gene function”)进一步检索。
HK4 recombinant protein is a engineered fusion protein designed for biomedical research and therapeutic applications. It typically combines functional domains derived from hepatitis B virus (HBV) core antigen (HBcAg) and other immunogenic or therapeutic components. The "HK4" designation often refers to a specific structural configuration featuring four tandem copies of the "immunodominant loop" (amino acids 76-83 of HBcAg), which is a key antigenic region that stimulates strong immune responses. This multimerization enhances antigen presentation and immune recognition compared to monomeric forms.
Originally developed for vaccine research, HK4's repetitive structure mimics viral surface epitopes, making it valuable in studying B-cell responses and developing virus-like particle (VLP) vaccines. Its self-assembling property allows formation of 30-35 nm nanoparticles resembling native HBV capsids, which can carry foreign epitopes or drug molecules. Recent applications extend beyond HBV studies, including as a carrier for heterologous antigens (e.g., malaria, HIV epitopes) and in targeted drug delivery systems due to its high stability and biocompatibility.
Production typically involves recombinant DNA technology in E. coli expression systems, followed by purification through ultracentrifugation and chromatography. The protein's cysteine-rich domains enable chemical conjugation for functional modifications. Current research explores its utility in cancer immunotherapy, particularly in delivering tumor-associated antigens, and as a diagnostic tool for monitoring HBV infection stages. Safety studies indicate low cytotoxicity, though immunogenicity requires careful evaluation in therapeutic contexts. Ongoing optimization focuses on enhancing thermal stability and loading capacity for diverse biomedical payloads.
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