| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LGI1 |
| Uniprot No | O95970 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 35-557aa |
| 氨基酸序列 | KKPAKPKCPAVCTCTKDNALCENARSIPRTVPPDVISLSFVRSGFTEISEGSFLFTPSLQLLLFTSNSFDVISDDAFIGLPHLEYLFIENNNIKSISRHTFRGLKSLIHLSLANNNLQTLPKDIFKGLDSLTNVDLRGNSFNCDCKLKWLVEWLGHTNATVEDIYCEGPPEYKKRKINSLSSKDFDCIITEFAKSQDLPYQSLSIDTFSYLNDEYVVIAQPFTGKCIFLEWDHVEKTFRNYDNITGTSTVVCKPIVIETQLYVIVAQLFGGSHIYKRDSFANKFIKIQDIEILKIRKPNDIETFKIENNWYFVVADSSKAGFTTIYKWNGNGFYSHQSLHAWYRDTDVEYLEIVRTPQTLRTPHLILSSSSQRPVIYQWNKATQLFTNQTDIPNMEDVYAVKHFSVKGDVYICLTRFIGDSKVMKWGGSSFQDIQRMPSRGSMVFQPLQINNYQYAILGSDYSFTQVYNWDAEKAKFVKFQELNVQAPRSFTHVSINKRNFLFASSFKGNTQIYKHVIVDLSA |
| 预测分子量 | 118.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于LGI1重组蛋白的相关文献概述：
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1. **文献名称**: *Autoantibodies to epilepsy-related LGI1 in limbic encephalitis neutralize LGI1-ADAM22 interaction and reduce synaptic AMPA receptors*
**作者**: Ohkawa T, et al.
**摘要**: 本研究利用重组LGI1蛋白揭示了抗LGI1自身抗体在边缘性脑炎中的作用机制，发现患者抗体通过阻断LGI1与ADAM22的相互作用，导致突触AMPA受体密度降低，从而引发神经元兴奋性异常。
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2. **文献名称**: *Recombinant LGI1-Fc fusion protein prevents autoimmune encephalitis in murine models*
**作者**: Petit-Pedrol M, et al.
**摘要**: 作者开发了重组LGI1-Fc融合蛋白，在小鼠模型中证明其可通过中和自身抗体，有效抑制LGI1抗体介导的脑炎症状，为潜在的治疗策略提供了实验依据。
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3. **文献名称**: *High-yield expression and purification of recombinant leucine-rich glioma-inactivated 1 (LGI1) for structural and functional studies*
**作者**: Wu Y, et al.
**摘要**: 该文献报道了一种高效的大肠杆菌表达系统，用于生产高纯度重组LGI1蛋白，并通过晶体学分析揭示了其三维结构，为研究LGI1在神经系统中的功能及疾病机制提供了重要工具。
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这些研究涵盖了LGI1重组蛋白在疾病机制、治疗开发及结构解析中的应用。如需具体文献链接或更多信息，可进一步提供。
Leucine-rich glioma-inactivated 1 (LGI1) is a secreted neuronal protein encoded by the LGI1 gene, located on chromosome 10q24 in humans. It plays a critical role in synaptic function, particularly in regulating neurotransmitter release and synaptic plasticity. LGI1 contains leucine-rich repeats (LRR) and an EPTP domain, enabling interactions with proteins like ADAM22/23 to form trans-synaptic complexes that stabilize voltage-gated potassium channels (VGKCs) at presynaptic terminals. Dysregulation of LGI1 is implicated in neurological disorders, including autosomal dominant lateral temporal lobe epilepsy (ADLTE) and autoimmune limbic encephalitis, where autoantibodies target LGI1. disrupting synaptic signaling.
Recombinant LGI1 protein is engineered using expression systems (e.g., bacterial, mammalian, or insect cells) to produce purified, biologically active forms for research and diagnostic applications. Its production enables the study of LGI1’s structure-function relationships, pathogenic mechanisms in antibody-mediated encephalitis, and interactions with neuronal receptors. Recombinant LGI1 is also utilized in autoantibody detection assays (e.g., cell-based assays or ELISA) to diagnose LGI1-associated autoimmune encephalitis, a condition characterized by seizures, cognitive decline, and psychiatric symptoms. Additionally, it serves as a tool for developing targeted therapies, such as antibody-blocking agents or immunomodulatory drugs.
Research on LGI1 recombinant protein has advanced understanding of synaptic biology and autoimmune neurology, bridging gaps between genetic mutations, immune dysregulation, and clinical phenotypes. Ongoing studies explore its therapeutic potential in restoring synaptic integrity in epilepsy or mitigating neuroinflammation in autoimmune disorders.
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