| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Entrez GeneID | 5565 |
| clone | 8F5B2 |
| WB Predicted band size | 30.3kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human PRKAB2 (AA: 1-120) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
+ +
以下是3-4篇与PRKAB2抗体相关的文献示例(注：文献信息为模拟示例，具体内容需根据实际文献调整)：
---
1. **标题**: "AMPK β2 subunit (PRKAB2) modulates tumor metabolism and chemoresistance in colorectal cancer"
**作者**: Smith A, et al.
**摘要**: 本研究通过Western blot和免疫组化分析，利用PRKAB2特异性抗体揭示了β2亚基在结直肠癌细胞能量代谢和化疗耐药中的作用，表明其表达水平与患者预后相关。
2. **标题**: "PRKAB2 regulates hepatic lipid metabolism through AMPK complex activation"
**作者**: Chen L, et al.
**摘要**: 使用PRKAB2抗体进行免疫沉淀和蛋白质互作分析，发现β2亚基在肝脏AMPK复合体组装中的关键功能，并证实其缺失导致小鼠脂代谢异常。
3. **标题**: "Tissue-specific expression profiling of AMPK subunits in diabetic models"
**作者**: Wang X, et al.
**摘要**: 通过PRKAB2抗体对糖尿病小鼠组织的Western blot和免疫荧光检测，揭示了β2亚基在骨骼肌和脂肪组织中的差异表达及其与胰岛素抵抗的关联。
4. **标题**: "PRKAB2 antibody-based screening identifies novel regulators of cardiac ischemia"
**作者**: Gonzalez R, et al.
**摘要**: 利用PRKAB2抗体筛选心脏缺血模型中的AMPK活性变化，发现β2亚基的磷酸化状态与心肌细胞能量应激反应的调控密切相关。
---
建议通过PubMed、Google Scholar或Web of Science等平台，以关键词“PRKAB2 antibody”或“AMPK β2 subunit”检索真实文献。部分研究可能侧重于抗体在疾病机制探索、蛋白质互作或亚细胞定位中的应用。
The PRKAB2 antibody targets the beta-2 subunit of AMP-activated protein kinase (AMPK), a critical energy sensor regulating cellular metabolism. AMPK is a heterotrimeric enzyme composed of α (catalytic), β, and γ subunits. The β-subunit (β1/PRKAB1 and β2/PRKAB2) acts as a scaffold, linking α and γ subunits, and influences AMPK stability, localization, and activity. PRKAB2 is encoded by the PRKAB2 gene on chromosome 1q21.2 and is predominantly expressed in heart, skeletal muscle, and liver. Unlike PRKAB1. PRKAB2 contains a carbohydrate-binding module, potentially modulating AMPK's interaction with glycogen.
Antibodies against PRKAB2 are essential tools for studying AMPK's role in metabolic pathways, including glucose uptake, fatty acid oxidation, and mitochondrial biogenesis. They are widely used in Western blotting, immunohistochemistry, and immunoprecipitation to assess PRKAB2 expression, localization, and protein interactions. Research applications span metabolic disorders (e.g., diabetes, obesity), cancer (due to AMPK's tumor-suppressive or pro-survival roles), and neurodegenerative diseases. PRKAB2-specific antibodies help distinguish β2-containing AMPK complexes from β1 isoforms, aiding investigations into tissue-specific AMPK functions. Dysregulation of PRKAB2 has been linked to insulin resistance, cardiac hypertrophy, and certain cancers, making these antibodies valuable for mechanistic and therapeutic studies. Validated antibodies typically confirm specificity via knockout controls or siRNA-mediated knockdown.
×
