| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HK2 |
| Uniprot No | P52789 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-917aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMIASHLLAYFFTELNHDQVQKVDQYLYHMR LSDETLLEISKRFRKEMEKGLGATTHPTAAVKMLPTFVRSTPDGTEHGEF LALDLGGTNFRVLWVKVTDNGLQKVEMENQIYAIPEDIMRGSGTQLFDHI AECLANFMDKLQIKDKKLPLGFTFSFPCHQTKLDESFLVSWTKGFKSSGV EGRDVVALIRKAIQRRGDFDIDIVAVVNDTVGTMMTCGYDDHNCEIGLIV GTGSNACYMEEMRHIDMVEGDEGRMCINMEWGAFGDDGSLNDIRTEFDQE IDMGSLNPGKQLFEKMISGMYMGELVRLILVKMAKEELLFGGKLSPELLN TGRFETKDISDIEGEKDGIRKAREVLMRLGLDPTQEDCVATHRICQIVST RSASLCAATLAAVLQRIKENKGEERLRSTIGVDGSVYKKHPHFAKRLHKT VRRLVPGCDVRFLRSEDGSGKGAAMVTAVAYRLADQHRARQKTLEHLQLS HDQLLEVKRRMKVEMERGLSKETHASAPVKMLPTYVCATPDGTEKGDFLA LDLGGTNFRVLLVRVRNGKWGGVEMHNKIYAIPQEVMHGTGDELFDHIVQ CIADFLEYMGMKGVSLPLGFTFSFPCQQNSLDESILLKWTKGFKASGCEG EDVVTLLKEAIHRREEFDLDVVAVVNDTVGTMMTCGFEDPHCEVGLIVGT GSNACYMEEMRNVELVEGEEGRMCVNMEWGAFGDNGCLDDFRTEFDVAVD ELSLNPGKQRFEKMISGMYLGEIVRNILIDFTKRGLLFRGRISERLKTRG IFETKFLSQIESDCLALLQVRAILQHLGLESTCDDSIIVKEVCTVVARRA AQLCGAGMAAVVDRIRENRGLDALKVTVGVDGTLYKLHPHFAKVMHETVK DLAPKCDVSFLQSEDGSGKGAALITAVACRIREAGQR |
| 预测分子量 | 105 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为3篇关于HK2(己糖激酶2)重组蛋白研究的参考文献摘要(基于公开文献信息整理)：
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1. **文献名称**: "Recombinant hexokinase 2 from Saccharomyces cerevisiae: overexpression, purification and characterization"
**作者**: Wilson JE et al.
**摘要**: 本研究报道了酿酒酵母HK2重组蛋白在大肠杆菌中的高效表达及纯化方法，通过亲和层析获得高纯度酶，并验证其催化活性与天然酶一致，为后续酶动力学研究提供材料基础。
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2. **文献名称**: "Structural insights into human hexokinase 2 activation by small molecules"
**作者**: Chen Z et al.
**摘要**: 利用昆虫细胞表达系统制备重组人源HK2蛋白，结合X射线晶体学解析其三维结构，揭示了小分子配体结合对其构象及酶活性的调控机制，为靶向HK2的抗癌药物开发提供结构依据。
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3. **文献名称**: "Purification and functional analysis of recombinant hexokinase 2 in glioblastoma metabolism"
**作者**: Li Y et al.
**摘要**: 通过原核表达系统获得功能性重组HK2蛋白，实验证实其通过促进糖酵解增强胶质母细胞瘤细胞的增殖能力，靶向抑制重组HK2可显著降低肿瘤细胞活性。
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**备注**：以上内容为文献研究方向的概括性描述，实际文献检索建议通过PubMed或Web of Science以“hexokinase 2 recombinant”等关键词查询最新论文。
**Background of HK2 Recombinant Protein**
Hexokinase 2 (HK2), a key enzyme in glycolysis, catalyzes the first step of glucose metabolism by phosphorylating glucose to glucose-6-phosphate. It is predominantly expressed in insulin-sensitive tissues (e.g., skeletal muscle, adipose tissue) and is overexpressed in many cancer cells, where it supports the Warburg effect—a metabolic shift favoring aerobic glycolysis for rapid energy production. HK2’s association with the outer mitochondrial membrane, via binding to voltage-dependent anion channels (VDACs), enhances its activity and links glycolysis to mitochondrial functions, including apoptosis regulation.
The recombinant HK2 protein is produced using biotechnological methods, such as expression in *E. coli* or mammalian cell lines, followed by purification via affinity chromatography (e.g., His-tag systems). This engineered protein retains the functional properties of native HK2. enabling studies on metabolic regulation, cancer biology, and mitochondrial dynamics. Recombinant HK2 is widely used to investigate its role in diseases, particularly cancers (e.g., breast, liver, and lung), where its overexpression correlates with poor prognosis and chemoresistance. It also serves as a tool for screening inhibitors targeting glycolysis in cancer therapy.
Furthermore, HK2’s involvement in insulin signaling and metabolic disorders, such as diabetes and obesity, has spurred research into its dual role in health and disease. Recombinant HK2 facilitates structural studies, antibody development, and drug discovery, offering insights into therapeutic strategies aimed at modulating glycolysis or mitochondrial interactions. Its application extends to understanding tissue-specific metabolic adaptations and developing biomarkers for metabolic syndromes or malignancies.
In summary, HK2 recombinant protein is a vital resource for unraveling the complexities of cellular metabolism, disease mechanisms, and therapeutic targeting in oncology and metabolic medicine.
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