| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HK1 |
| Uniprot No | P19367 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 13-475aa |
| 氨基酸序列 | ELKDDQVKKIDKYLYAMRLSDETLIDIMTRFRKEMKNGLSRDFNPTATVKMLPTFVRSIPDGSEKGDFIALDLGGSSFRILRVQVNHEKNQNVHMESEVYDTPENIVHGSGSQLFDHVAECLGDFMEKRKIKDKKLPVGFTFSFPCQQSKIDEAILITWTKRFKASGVEGADVVKLLNKAIKKRGDYDANIVAVVNDTVGTMMTCGYDDQHCEVGLIIGTGTNACYMEELRHIDLVEGDEGRMCINTEWGAFGDDGSLEDIRTEFDREIDRGSLNPGKQLFEKMVSGMYLGELVRLILVKMAKEGLLFEGRITPELLTRGKFNTSDVSAIEKNKEGLHNAKEILTRLGVEPSDDDCVSVQHVCTIVSFRSANLVAATLGAILNRLRDNKGTPRLRTTVGVDGSLYKTHPQYSRRFHKTLRRLVPDSDVRFLLSESGSGKGAAMVTAVAYRLAEQHRQIEETLA |
| 预测分子量 | 55.9kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HK1(Hexokinase 1)重组蛋白的3篇参考文献示例，内容基于真实研究整理：
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1. **文献名称**: "Expression and Purification of Recombinant Human Hexokinase 1 for Structural Studies"
**作者**: Smith J, et al.
**摘要**: 该研究描述了人源HK1重组蛋白在大肠杆菌中的高效表达与纯化方法，通过X射线晶体学分析其三维结构，揭示了其底物结合域的关键构象变化，为靶向HK1的抗肿瘤药物设计提供依据。
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2. **文献名称**: "Functional Characterization of Recombinant HK1 in Cancer Cell Glycolysis"
**作者**: Li X, Wang Y.
**摘要**: 研究团队通过哺乳动物细胞系统表达HK1重组蛋白，证明其在肿瘤细胞线粒体膜上的异常定位显著增强糖酵解活性，并验证了HK1与VDAC蛋白的相互作用在肿瘤代谢重编程中的作用。
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3. **文献名称**: "Development of a Novel HK1-Based Biosensor for Glucose Monitoring"
**作者**: Chen L, et al.
**摘要**: 利用重组HK1蛋白与荧光探针结合，开发了一种高灵敏度葡萄糖检测生物传感器，通过体外实验验证了其在低浓度葡萄糖环境下的稳定性和特异性，可能用于糖尿病诊断技术优化。
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注：以上文献信息为模拟示例，实际引用需查询具体数据库(如PubMed、Web of Science)获取真实发表记录。
**Background of HK1 Recombinant Protein**
Hexokinase 1 (HK1) is a critical enzyme in glycolysis, catalyzing the first step of glucose metabolism by phosphorylating glucose to glucose-6-phosphate. As a member of the hexokinase family, HK1 is ubiquitously expressed, with high activity in tissues reliant on rapid glucose utilization, such as the brain, muscle, and red blood cells. Unlike other isoforms (e.g., HK2), HK1 is constitutively expressed and anchored to the mitochondrial outer membrane, where it couples glycolysis to mitochondrial ATP production, influencing cellular energy homeostasis and apoptosis regulation.
Recombinant HK1 protein is produced using biotechnological methods, such as expression in *E. coli* or mammalian cell systems, followed by purification to ensure high specificity and activity. This engineered protein retains the functional domains of native HK1. including the glucose-binding site and regulatory regions, making it invaluable for *in vitro* studies. Researchers employ recombinant HK1 to investigate metabolic dysregulation in diseases like cancer (where glycolytic flux is often elevated), diabetes, and neurodegenerative disorders. It also serves as a tool for screening potential inhibitors or activators targeting hexokinase activity.
HK1's role in cellular metabolism and its implications in pathologies have driven interest in its structural and functional characterization. Recombinant HK1 enables detailed enzymatic assays, structural studies (e.g., X-ray crystallography), and exploration of post-translational modifications affecting its activity. Furthermore, its mitochondrial association has sparked research into HK1's non-metabolic functions, such as modulating oxidative stress and cell death pathways. By providing a controlled and scalable source of HK1. recombinant technology accelerates both basic research and therapeutic development, highlighting its significance in biomedical sciences.
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