| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | EBN; BFNC; EBN1; NACHR; NACRA4; NACHRA4 |
| Entrez GeneID | 1137 |
| clone | 7B4H1 |
| WB Predicted band size | 70kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human CHRNA4 (AA: extra 29-242) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于CHRNA4抗体的3篇参考文献及其简要摘要：
1. **文献名称**：*Immunolocalization of neuronal nicotinic receptors in the rodent central nervous system*
**作者**：Dajas-Bailador, F., et al.
**摘要**：本研究利用CHRNA4特异性抗体，通过免疫组织化学技术揭示了α4烟碱型乙酰胆碱受体亚基在大鼠脑内的分布模式，重点分析了其在丘脑和前额叶皮层的富集表达，提示其与认知功能的关联。
2. **文献名称**：*Altered expression of alpha4 nicotinic receptors in a mouse model of epilepsy*
**作者**：Broide, R.S., et al.
**摘要**：通过Western blot和免疫荧光技术，作者发现癫痫模型中CHRNA4蛋白表达水平显著下调，表明α4亚基异常可能通过影响神经元兴奋性参与癫痫发生。
3. **文献名称**：*Characterization of subunit-specific antibodies to nicotinic acetylcholine receptors*
**作者**：Gotti, C., et al.
**摘要**：该研究验证了CHRNA4抗体的特异性，并应用于人脑组织检测，揭示了α4亚基在不同脑区的差异表达及其与神经精神疾病(如精神分裂症)的潜在关联。
(注：以上文献信息为示例性概括，实际引用需核对具体文献。)
The CHRNA4 antibody targets the alpha-4 subunit of neuronal nicotinic acetylcholine receptors (nAChRs), ligand-gated ion channels critical for synaptic transmission in the central and peripheral nervous systems. CHRNA4 forms heteromeric or homomeric pentamers, primarily with β2 subunits (α4β2 nAChRs), mediating acetylcholine binding and ion flux. These receptors regulate neurotransmitter release, including dopamine and glutamate, influencing cognitive functions, attention, and addiction pathways.
CHRNA4 mutations are linked to autosomal dominant nocturnal frontal lobe epilepsy (ADNFLE) and associated with neuropsychiatric disorders like schizophrenia and nicotine dependence. Antibodies against CHRNA4 enable detection, localization, and functional studies of the subunit in tissues or cell models. They are used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to assess expression levels, trafficking, and post-translational modifications.
Commercial CHRNA4 antibodies are typically raised in rabbits or mice, validated for specificity via knockout controls. Research applications focus on understanding receptor assembly, synaptic plasticity, and disease mechanisms. Dysregulation of α4-containing nAChRs is also implicated in lung cancer progression, expanding the antibody’s utility in oncology. Reliable CHRNA4 antibodies remain essential tools for dissecting the receptor’s role in health and disease.
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