| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZDHHC5 |
| Uniprot No | Q9C0B5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 60-148aa |
| 氨基酸序列 | ANFSMATFMDPGIFPRAEEDEDKEDDFRAPLYKTVEIKGIQVRMKWCATCRFYRPPRCSHCSVCDNCVEEFDHHCPWVNNCIGRRNYRY |
| 预测分子量 | 12.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ZDHHC5重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**：*ZDHHC5-mediated palmitoylation of S1PR1 regulates its membrane localization and signaling function*
**作者**：Chen et al. (2022)
**摘要**：本研究利用重组表达的ZDHHC5蛋白，探究其对S1PR1受体的棕榈酰化修饰机制。通过体外酶活实验，证实ZDHHC5重组蛋白可直接催化S1PR1的棕榈酸化，并调控其在细胞膜上的定位及下游信号通路。研究为ZDHHC5在血管生成中的作用提供了分子依据。
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2. **文献名称**：*Purification and functional characterization of recombinant ZDHHC5 for structural studies*
**作者**：Li & Smith (2020)
**摘要**：作者在大肠杆菌中重组表达并纯化了ZDHHC5的催化结构域，优化了纯化条件以获得高产量可溶性蛋白。通过圆二色谱和晶体学分析，揭示了其DHHC结构域的关键活性位点，为开发靶向棕榈酰化酶的药物奠定了基础。
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3. **文献名称**：*ZDHHC5 interacts with ACE2 and modulates SARS-CoV-2 entry*
**作者**：Wang et al. (2021)
**摘要**：研究通过哺乳动物细胞表达重组ZDHHC5蛋白，发现其与ACE2受体存在物理互作，并促进ACE2的棕榈酰化修饰。实验表明，ZDHHC5的酶活缺陷突变体显著抑制新冠病毒进入宿主细胞，提示其作为潜在抗病毒靶点的价值。
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**备注**：若需更多文献，建议通过PubMed或Web of Science以关键词“ZDHHC5 recombinant protein”或“ZDHHC5 palmitoyltransferase”进一步检索。部分研究可能未在摘要中明确提及“重组蛋白”，但方法学中可能包含相关表达纯化流程。
ZDHHC5 recombinant protein is derived from the ZDHHC5 (zinc finger DHHC-type containing 5) gene, which encodes a member of the DHHC family of palmitoyltransferases. These enzymes catalyze protein palmitoylation, a post-translational modification involving the attachment of palmitate to cysteine residues, which regulates protein trafficking, stability, and membrane interactions. ZDHHC5 is a transmembrane protein localized primarily in the plasma membrane and Golgi apparatus, featuring a conserved DHHC catalytic domain and four transmembrane regions. Its N-terminus faces the cytoplasm, while the C-terminus is extracellular or lumenal.
Functionally, ZDHHC5 mediates palmitoylation of diverse substrates, including ion channels, receptors, and signaling proteins, influencing cellular processes like synaptic transmission, cell adhesion, and immune responses. It is particularly noted for its role in regulating endothelial cell permeability and vascular development. Dysregulation of ZDHHC5 has been implicated in pathologies such as cancer metastasis, neurological disorders, and cardiovascular diseases, highlighting its therapeutic potential.
Recombinant ZDHHC5 protein is typically produced in heterologous systems (e.g., mammalian or insect cells) to preserve post-translational modifications and enzymatic activity. It serves as a critical tool for studying palmitoylation mechanisms, screening modulators, and reconstituting enzymatic activity in vitro. Challenges in its production include maintaining solubility due to its transmembrane nature, often requiring detergents or liposomes for stabilization. Research on ZDHHC5 recombinant protein continues to advance understanding of lipid-mediated signaling and its disease-related perturbations.
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