| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | aprA |
| Uniprot No | P04189 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 107-381aa |
| 氨基酸序列 | AQSVPYGISQIKAPALHSQGYTGSNVKVAVIDSGIDSSHPDLNVRGGASFVPSETNPYQDGSSHGTHVAGTIAALNNSIGVLGVAPSASLYAVKVLDSTGSGQYSWIINGIEWAISNNMDVINMSLGGPTGSTALKTVVDKAVSSGIVVAAAAGNEGSSGSTSTVGYPAKYPSTIAVGAVNSSNQRASFSSAGSELDVMAPGVSIQSTLPGGTYGAYNGTSMATPHVAGAAALILSKHPTWTNAQVRDRLESTATYLGNSFYYGKGLINVQAAAQ |
| 预测分子量 | 43.7kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与aprA重组蛋白相关的文献示例(内容为虚构模拟，仅供参考格式)：
1. **"Cloning and Expression of the aprA Gene from Pseudomonas aeruginosa in E. coli"**
- Author: Smith J, et al.
- 摘要：研究通过PCR扩增铜绿假单胞菌的aprA基因，构建重组质粒并在大肠杆菌中表达，证实重组蛋白具有蛋白酶活性，为后续功能研究奠定基础。
2. **"Structural Characterization of Recombinant AprA Protease and Its Role in Bacterial Pathogenesis"**
- Author: Lee H, et al.
- 摘要：利用X射线晶体学解析重组AprA蛋白的三维结构，揭示其活性位点特征，并通过体外实验证明该蛋白酶在宿主组织降解中的关键作用。
3. **"Optimization of aprA Recombinant Protein Production Using a Fed-Batch Fermentation System"**
- Author: Chen R, et al.
- 摘要：通过优化培养条件和诱导策略，显著提高重组AprA蛋白在大肠杆菌中的产量，为工业化制备提供技术参考。
4. **"Functional Analysis of aprA Mutants in Biofilm Formation"**
- Author: García M, et al.
- 摘要：构建aprA点突变重组蛋白，发现特定氨基酸残基缺失会削弱铜绿假单胞菌生物膜形成能力，提示AprA在细菌群体行为中的调控功能。
**注**：以上文献信息为示例性模拟，实际研究中需通过PubMed、Google Scholar等平台检索真实文献。建议使用关键词"aprA recombinant protein"、"Pseudomonas aeruginosa aprA expression"进行查询。
Here’s a concise description of aprA recombinant protein in English, formatted naturally under 400 words:
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The **aprA** gene encodes an extracellular protease known as alkaline protease A, primarily studied in *Pseudomonas aeruginosa*, a Gram-negative opportunistic pathogen. This metalloprotease contributes to bacterial virulence by degrading host proteins such as collagen, elastin, and immune factors, facilitating tissue invasion and immune evasion. aprA is regulated by quorum sensing, linking its production to bacterial population density during infections.
Recombinant aprA protein is produced via genetic engineering, where the aprA gene is cloned into expression vectors (e.g., *E. coli* or yeast systems) to enable large-scale protein synthesis. Purification techniques like affinity chromatography yield high-purity aprA for functional studies. Researchers utilize recombinant aprA to dissect its enzymatic mechanisms, substrate specificity, and role in pathogenesis. It also serves as a target for developing inhibitors or antibodies to combat antibiotic-resistant infections.
Beyond pathogenicity, aprA’s proteolytic activity has industrial applications, including waste processing and bio-detergent formulations. Structural studies of recombinant aprA provide insights into its zinc-binding catalytic domain and potential allosteric sites for modulation. However, challenges remain in maintaining its stability and activity *in vitro*, necessitating optimized expression conditions.
In biotechnology, aprA recombinant protein is a tool for studying protease-driven processes in microbial communities and host-pathogen interactions. Its relevance to biofilm formation and chronic infections underscores its importance in both clinical and environmental microbiology research.
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This overview covers aprA’s biological role, production methods, research applications, and broader significance. Let me know if you need further elaboration!
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