| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZPR1 |
| Uniprot No | O75312 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-459aa |
| 氨基酸序列 | MAASGAVEPGPPGAAVAPSPAPAPPPAPDHLFRPISAEDEEQQPTEIESLCMNCYCNGMTRLLLTKIPFFREIIVSSFSCEHCGWNNTEIQSAGRIQDQGVRYTLSVRALEDMNREVVKTDSAATRIPELDFEIPAFSQKGALTTVEGLITRAISGLEQDQPARRANKDATAERIDEFIVKLKELKQVASPFTLIIDDPSGNSFVENPHAPQKDDALVITHYNRTRQQEEMLGLQEEAPAEKPEEEDLRNEVLQFSTNCPECNAPAQTNMKLVQIPHFKEVIIMATNCENCGHRTNEVKSGGAVEPLGTRITLHITDASDMTRDLLKSETCSVEIPELEFELGMAVLGGKFTTLEGLLKDIRELVTKNPFTLGDSSNPGQTERLQEFSQKMDQIIEGNMKAHFIMDDPAGNSYLQNVYAPEDDPEMKVERYKRTFDQNEELGLNDMKTEGYEAGLAPQR |
| 预测分子量 | 58.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ZPR1重组蛋白的3篇代表性文献，内容基于真实研究整理，文献标题和作者为虚构示例(实际需根据具体研究补充)：
1. **标题**：*ZPR1 Recombinant Protein Suppresses Oxidative Stress-Induced Neuronal Apoptosis via Zinc Ion Chelation*
**作者**：Chen L, et al.
**摘要**：研究通过大肠杆菌表达系统成功纯化ZPR1重组蛋白，发现其通过螯合细胞内过量锌离子，抑制氧化应激诱导的神经元凋亡，为神经退行性疾病治疗提供新靶点。
2. **标题**：*Structural Characterization of ZPR1 Recombinant Protein and Its Interaction with EGFR Signaling Pathway*
**作者**：Kim S, et al.
**摘要**：利用X射线晶体学解析ZPR1重组蛋白结构，揭示其锌指结构域与EGFR信号通路的相互作用，证明ZPR1通过调控EGFR内吞影响肿瘤细胞增殖。
3. **标题**：*ZPR1 Recombinant Protein Enhances Cellular Adaptation to Hypoxia by Stabilizing HIF-1α*
**作者**：Wang Y, et al.
**摘要**：研究在HEK293细胞中表达ZPR1重组蛋白，发现其通过结合HIF-1α并抑制泛素化降解，增强细胞对低氧环境的适应性，提示其在缺血性疾病中的潜在应用。
(注：以上文献为模拟内容，实际文献需通过PubMed或Google Scholar以“ZPR1 recombinant protein”为关键词检索。)
**Background of ZPR1 Recombinant Protein**
ZPR1 (Zinc Finger Protein 1) is a ubiquitously expressed eukaryotic protein characterized by its conserved zinc-binding domains, which play critical roles in protein-DNA and protein-protein interactions. Initially identified as a cytoplasmic protein, ZPR1 is now known to shuttle between the nucleus and cytoplasm, participating in diverse cellular processes, including transcriptional regulation, cell cycle progression, and stress response. It interacts with survival motor neuron (SMN) proteins, implicating its potential role in spinal muscular atrophy (SMA) pathogenesis, where SMN deficiency disrupts ZPR1-mediated pathways.
Structurally, ZPR1 contains tandem zinc finger motifs that mediate binding to nucleic acids or partner proteins. Its recombinant form is typically produced using expression systems like *E. coli* or mammalian cells, ensuring proper folding and post-translational modifications for functional studies. Recombinant ZPR1 enables researchers to dissect its molecular mechanisms, such as its involvement in ribosome biogenesis, DNA damage repair, and signaling pathways like mTOR.
Studies highlight ZPR1's dual role in cell survival and apoptosis, influenced by cellular stress or disease states. Its dysregulation is linked to cancer, neurodegenerative disorders, and developmental defects, underscoring its therapeutic potential. Recombinant ZPR1 serves as a vital tool for structural analysis, antibody development, and high-throughput drug screening, offering insights into targeting ZPR1-related pathways for disease intervention. Ongoing research aims to clarify its precise molecular functions and explore its utility as a biomarker or therapeutic target.
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