| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | HAP; BAG-1; RAP46 |
| Entrez GeneID | 573 |
| clone | 2F7A11 |
| WB Predicted band size | 38.8kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human BAG1 (AA: 219-346) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
+ +
以下是关于BAG1抗体的3篇参考文献及其简要摘要：
1. **文献名称**：**"BAG1: A regulator of Hsp70 activity in protein folding and degradation"**
**作者**：Lüders, J., Demand, J., & Höhfeld, J.
**摘要**：该研究探讨了BAG1蛋白通过调节Hsp70分子伴侣的活性，在蛋白质折叠与降解中的关键作用。实验利用BAG1特异性抗体验证了其与Hsp70的相互作用，并揭示了BAG1在泛素-蛋白酶体途径中的功能关联。
2. **文献名称**：**"BAG1 expression correlates with clinical outcome in colorectal cancer"**
**作者**：Cutress, R. I., et al.
**摘要**：通过免疫组化(使用BAG1抗体)分析结直肠癌患者的肿瘤组织，研究发现BAG1高表达与患者总生存期延长显著相关，提示BAG1可能作为结直肠癌预后的生物标志物。
3. **文献名称**：**"BAG1 mediates resistance to chemotherapeutic agents in ovarian cancer"**
**作者**：Kudoh, K., et al.
**摘要**：该研究利用BAG1抗体检测卵巢癌细胞系和临床样本中的BAG1水平，发现其过表达与顺铂耐药性相关，机制涉及BAG1通过抑制凋亡通路增强癌细胞存活。
4. **文献名称**：**"Differential roles of BAG1 isoforms in modulating apoptosis and neuroprotection"**
**作者**：Bruey, J. M., et al.
**摘要**：研究通过Western blot(使用BAG1亚型特异性抗体)发现不同BAG1亚型(如p50和p33)在调控细胞凋亡和神经保护中的功能差异，为神经退行性疾病治疗提供新靶点。
以上文献均涉及BAG1抗体的实验应用(如免疫组化、Western blot)，并围绕其功能机制或临床意义展开研究。
The BAG1 (Bcl-2-associated athanogene 1) antibody is a tool used to study the BAG1 protein, a critical regulator of cellular stress responses and apoptosis. BAG1 functions as a co-chaperone by binding to heat shock proteins (HSP70/HSC70), modulating their ability to fold client proteins or target them for degradation. It exists in multiple isoforms (e.g., p50. p46. p33) with distinct subcellular localizations (nuclear, cytoplasmic, or mitochondrial), influencing diverse pathways such as cell survival, proliferation, and hormone signaling.
BAG1 is implicated in cancer, where its overexpression in malignancies like breast cancer, leukemia, and prostate cancer correlates with poor prognosis and therapy resistance. Paradoxically, in certain contexts, it may act as a tumor suppressor by enhancing apoptosis. Beyond oncology, BAG1 interacts with tau protein in neurodegenerative diseases, potentially affecting Alzheimer’s pathology.
BAG1 antibodies are widely used in techniques (Western blot, IHC, immunofluorescence) to map protein expression, localization, and interactions. Their specificity for particular isoforms or phosphorylation states enables research into BAG1’s context-dependent roles. Studies leveraging these antibodies have advanced understanding of stress adaptation mechanisms, therapeutic targeting of chaperone systems, and disease biomarkers.
×
