| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DNAJC5 |
| Uniprot No | Q9H3Z4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-198aa |
| 氨基酸序列 | MADQRQRSLSTSGESLYHVLGLDKNATSDDIKKSYRKLALKYHPDKNPDNPEAADKFKEINNAHAILTDATKRNIYDKYGSLGLYVAEQFGEENVNTYFVLSSWWAKALFVFCGLLTCCYCCCCLCCCFNCCCGKCKPKAPEGEETEFYVSPEDLEAQLQSDEREATDTPIVIQPASATETTQLTADSHPSYHTDGFN |
| 预测分子量 | 69.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DNAJC5重组蛋白的3篇代表性文献示例(注：文献信息为模拟概括，仅供参考)：
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1. **标题**：*DNAJC5 Facilitates α-Synuclein Clearance through Lysosomal Degradation Pathways*
**作者**：Zhang L, et al.
**摘要**：研究通过重组DNAJC5蛋白在HEK293细胞中过表达，证实其作为分子伴侣与α-突触核蛋白相互作用，增强错误折叠蛋白通过溶酶体自噬途径的降解，可能为帕金森病治疗提供靶点。
2. **标题**：*Recombinant DNAJC5 Protein Modulates Synaptic Vesicle Trafficking in Neuronal Models*
**作者**：Fernandez-Ruiz I, et al.
**摘要**：利用重组DNAJC5蛋白进行体外囊泡结合实验，发现其通过调控突触囊泡的运输和膜融合过程，维持神经递质释放稳态，功能缺失可能导致突触功能障碍。
3. **标题**：*Structural and Functional Analysis of DNAJC5 Interaction with Hsc70*
**作者**：Wang Q, et al.
**摘要**：通过大肠杆菌表达纯化重组DNAJC5蛋白，结合晶体学分析揭示其C端结构域与Hsc70的协同作用机制，为DNAJ家族蛋白的分子伴侣功能提供结构基础。
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**说明**：DNAJC5(又称CSPα)属于热休克蛋白Hsp40/DNAJ家族，广泛参与神经细胞中蛋白质质量控制。上述模拟研究聚焦其重组蛋白在疾病关联蛋白降解、囊泡运输及分子伴侣机制中的功能，实际文献需通过学术数据库检索确认。
DNAJC5. also known as cysteine string protein alpha (CSPα), is a member of the J-protein family that functions as a co-chaperone for heat shock protein 70 (HSP70). It plays a critical role in neuronal maintenance, synaptic vesicle cycling, and protein quality control. Structurally, it contains a conserved J-domain for HSP70 interaction, a cysteine-rich "string" region involved in membrane association, and a C-terminal domain for binding client proteins. Notably, DNAJC5 undergoes palmitoylation at its cysteine residues, enabling attachment to synaptic vesicles and lysosomal membranes.
Recombinant DNAJC5 proteins are engineered to study its molecular mechanisms and disease associations. Mutations in DNAJC5 (e.g., Leu115Arg or Leu116Δ) are linked to adult-onset neuronal ceroid lipofuscinosis (ANCL), a rare neurodegenerative disorder characterized by lysosomal dysfunction and abnormal protein aggregation. Recombinant forms allow researchers to investigate how these mutations impair CSPα's chaperone activity, disrupt synaptic transmission, or promote toxic oligomerization.
Production typically involves expression systems like E. coli or mammalian cells, followed by purification via affinity tags. These recombinant tools are used in in vitro assays to map protein interactions (e.g., with HSP70. SGTα, or synaptic proteins like SNARE components), analyze palmitoylation dynamics, and model pathological aggregation. Recent studies also explore DNAJC5's potential role in exosome secretion and autophagy, highlighting its broader implications in proteostasis. Therapeutic strategies targeting DNAJC5 pathways, including HSP70 enhancers or anti-aggregation molecules, are under investigation for neurodegenerative diseases.
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