| 纯度 | >90%SDS-PAGE. |
| 种属 | Mouse |
| 靶点 | Cyp3a11 |
| Uniprot No | Q64459 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-504aa |
| 氨基酸序列 | MDLVSALSLETWVLLAISLVLLYRYGTRKHELFKKQGIPGPKPLPFLGTVLNYYKGLWKFDMECYKKYGKTWGLFDGQTPLLAVTDPETIKNVLVKECFSVFTNRRDFGPVGIMSKAISISKDDEWKRYRALLSPTFTSGKLKEMFPVIEQYGDILVKYLRQKAKKGKPVTMKDVLGAYSMDVITSTSFGVNVDSLNNPEDPFVEKAKKLLRFDFFDPLLFSVVLFPFLTPVYEMLNICMFPKDSIEFFKKFVDRMKESRLDSKQKHRVDFLQLMMNSHNNSKDKVSHKALSDMEITAQSIIFIFAGYETTSSTLSFTLHSLATHPDIQKKLQDEIDEALPNKAPPTYDTVMEMEYLDMVLNETLRLYPIANRLERVCKKDVELNGVYIPKGSTVMIPSYALHHDPQHWSEPEEFQPERFSKENKGSIDPYVYLPFGNGPRNCLGMRFALMNMKLALTKIMQNFSFQPCKETQIPLKLSRQGLLQPEKPIVLKVVPRDAVITGA |
| 预测分子量 | 65.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于Cyp3a11重组蛋白的3篇参考文献及其摘要概括：
1. **文献名称**：*"Functional characterization of recombinant Cyp3a11 expressed in baculovirus-infected insect cells"*
**作者**：Zhang H. et al.
**摘要**：本研究利用杆状病毒-昆虫细胞系统成功表达了小鼠Cyp3a11重组蛋白，并通过光谱分析和体外代谢实验验证其酶活性。结果表明，重组Cyp3a11可高效代谢睾酮和咪达唑仑，为药物代谢研究提供了可靠的体外模型。
2. **文献名称**：*"Comparison of Escherichia coli and mammalian expression systems for Cyp3a11 production"*
**作者**：Imaoka S. et al.
**摘要**：作者比较了大肠杆菌与哺乳动物细胞(HEK293)表达系统对Cyp3a11重组蛋白的产量及功能影响。结果显示，哺乳动物系统表达的蛋白具有更高的催化活性和稳定性，但大肠杆菌系统成本更低，适用于部分基础研究。
3. **文献名称**：*"Role of Cyp3a11 in drug-drug interactions: Insights from recombinant enzyme assays"*
**作者**：Yamazaki H. et al.
**摘要**：通过重组Cyp3a11蛋白评估了多种药物(如利福平、酮康唑)对其活性的诱导或抑制作用，揭示了Cyp3a11在小鼠药物相互作用中的关键代谢途径，为临床前药物开发提供参考。
4. **文献名称**：*"Cyp3a11 knockout mice and recombinant protein rescue: Implications for xenobiotic metabolism"*
**作者**：Matsubara T. et al.
**摘要**：研究利用Cyp3a11基因敲除小鼠模型，结合外源性重组Cyp3a11蛋白回补实验，证实该酶在体内外源性物质清除中的必要性，并探讨了其底物特异性与人类CYP3A4的差异。
(注：以上文献信息为基于领域知识的模拟示例，实际引用请以具体文献为准。)
Cyp3a11. a member of the cytochrome P450 3A (CYP3A) subfamily, is a mouse ortholog of human CYP3A4. one of the most critical drug-metabolizing enzymes in mammals. This heme-containing monooxygenase is predominantly expressed in the liver and small intestine, where it plays a central role in the oxidative metabolism of diverse endogenous and exogenous compounds, including pharmaceuticals, steroids, and environmental toxins. Cyp3a11-mediated reactions, such as hydroxylation, dealkylation, and epoxidation, contribute significantly to phase I metabolism, influencing drug bioavailability, clearance, and potential drug-drug interactions.
Recombinant Cyp3a11 protein is engineered to facilitate in vitro studies of drug metabolism and enzyme kinetics without requiring tissue extracts or live animal models. It is commonly produced using heterologous expression systems like baculovirus-infected insect cells (e.g., Sf9 cells) or mammalian cell lines, often with modifications such as N-terminal truncations or affinity tags (e.g., His-tag) to enhance solubility and purification efficiency. The recombinant protein retains catalytic activity comparable to native Cyp3a11. enabling researchers to assess substrate specificity, inhibitory effects, and interspecies metabolic differences, particularly in preclinical drug development.
Studies using recombinant Cyp3a11 have provided insights into murine-specific metabolic pathways, aiding the interpretation of pharmacokinetic data from mouse models. Its application extends to investigating enzyme induction/repression mechanisms, polymorphisms, and interactions with nuclear receptors (e.g., PXR, CAR). As mice lacking Cyp3a11 (Cyp3a-knockout models) are used to mimic human CYP3A4-related drug responses, the recombinant protein serves as a vital tool for bridging in vitro and in vivo findings in pharmacology and toxicology research.
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