| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TRPC4AP |
| Uniprot No | Q8TEL6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-797aa |
| 氨基酸序列 | AAAPVAAGSGAGRGRRSAATVAAWGGWGGRPRPGNILLQLRQGQLTGRGLVRAVQFTETFLTERDKQSKWSGIPQLLLKLHTTSHLHSDFVECQNILKEISPLLSMEAMAFVTEERKLTQETTYPNTYIFDLFGGVDLLVEILMRPTISIRGQKLKISDEMSKDCLSILYNTCVCTEGVTKRLAEKNDFVIFLFTLMTSKKTFLQTATLIEDILGVKKEMIRLDEVPNLSSLVSNFDQQQLANFCRILAVTISEMDTGNDDKHTLLAKNAQQKKSLSLGPSAAEINQAALLSIPGFVERLCKLATRKVSESTGTASFLQELEEWYTWLDNALVLDALMRVANEESEHNQASIVFPPPGASEENGLPHTSARTQLPQSMKIMHEIMYKLEVLYVLCVLLMGRQRNQVHRMIAEFKLIPGLNNLFDKLIWRKHSASALVLHGHNQNCDCSPDITLKIQFLRLLQSFSDHHENKYLLLNNQELNELSAISLKANIPEVEAVLNTDRSLVCDGKRGLLTRLLQVMKKEPAESSFRFWQARAVESFLRGTTSYADQMFLLKRGLLEHILYCIVDSECKSRDVLQSYFDLLGELMKFNVDAFKRFNKYINTDAKFQVFLKQINSSLVDSNMLVRCVTLSLDRFENQVDMKVAEVLSECRLLAYISQVPTQMSFLFRLINIIHVQTLTQENVSCLNTSLVILMLARRKERLPLYLRLLQRMEHSKKYPGFLLNNFHNLLRFWQQHYLHKDKDSTCLENSSCISFSYWKETVSILLNPDRQSPSALVSYIEEPYMDIDRDFTEE |
| 预测分子量 | 94.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与TRPC4AP重组蛋白相关的参考文献摘要：
1. **《TRPC4AP interacts with STIM1 to mediate store-operated calcium entry in endothelial cells》**
- 作者：Chen, X. et al. (2019)
- 摘要：研究利用重组TRPC4AP蛋白揭示了其通过结合STIM1调控内皮细胞钙库操纵性钙内流(SOCE)的分子机制，为血管功能研究提供新靶点。
2. **《Expression and purification of functional human TRPC4AP in HEK293 cells》**
- 作者：Wang, L. & Zhang, Y. (2017)
- 摘要：报道了人源TRPC4AP重组蛋白在HEK293细胞中的优化表达与纯化流程，证实其具有结合TRPC4离子通道并增强其活性的功能。
3. **《TRPC4AP modulates β-catenin signaling in colorectal cancer via calcium-dependent pathways》**
- 作者：Kim, S. et al. (2021)
- 摘要：通过重组TRPC4AP蛋白实验发现，该蛋白通过钙信号通路影响β-catenin活性，可能成为结直肠癌治疗的潜在干预靶点。
注：以上文献信息为示例性质，实际引用前建议通过PubMed/Google Scholar核实具体研究。若需精确文献，可补充提供研究方向(如蛋白纯化/疾病机制)。
TRPC4AP (Transient Receptor Potential Cation Channel Subfamily C Member 4-Associated Protein), also known as TRUSS or C4AP, is a scaffold protein implicated in diverse cellular processes, including calcium signaling, transcriptional regulation, and apoptosis. It was initially identified through its interaction with TRPC4. a member of the TRP channel family that mediates calcium influx in response to G protein-coupled receptor (GPCR) activation. TRPC4AP contains multiple protein interaction domains, such as coiled-coil regions, enabling it to serve as a molecular bridge between signaling molecules.
This protein plays a dual role in cellular homeostasis. It regulates calcium entry by modulating TRPC4 channel activity, influencing downstream pathways like NF-κB and MAPK, which are critical for cell proliferation and inflammatory responses. Additionally, TRPC4AP interacts with transcription factors (e.g., STAT3) and RNA-binding proteins, suggesting involvement in gene expression regulation. Paradoxically, TRPC4AP can promote apoptosis under stress conditions by enhancing JNK signaling, while also exhibiting oncogenic properties in certain cancers through survival pathway activation.
Recombinant TRPC4AP proteins are engineered for functional studies, typically expressed in bacterial or mammalian systems with affinity tags (e.g., His or GST tags) for purification. These tools enable researchers to dissect TRPC4AP’s structural domains, map interaction networks, and screen for therapeutic modulators. Dysregulation of TRPC4AP has been linked to cancer, cardiovascular diseases, and neurological disorders, making it a potential biomarker or drug target. Ongoing research aims to clarify its context-dependent roles and therapeutic relevance.
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