| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HERC5 |
| Uniprot No | Q9UII4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 681-1024aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSFDLTVRRNHLIEDVLNQLSQFENEDLRKELWVSFSGEIGYDLGGVKK EFFYCLFAEMIQPEYGMFMYPEGASCMWFPVKPKFEKKRYFFFGVLCGLS LFNCNVANLPFPLALFKKLLDQMPSLEDLKELSPDLGKNLQTLLDDEGDN FEEVFYIHFNVHWDRNDTNLIPNGSSITVNQTNKRDYVSKYINYIFNDSV KAVYEEFRRGFYKMCDEDIIKLFHPEELKDVIVGNTDYDWKTFEKNARYE PGYNSSHPTIVMFWKAFHKLTLEEKKKFLVFLTGTDRLQMKDLNNMKITF CCPESWNERDPIRALTCFSVLFLPKYSTMETVEEALQEAINNNRGFG |
| 预测分子量 | 43 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HERC5重组蛋白的3篇示例参考文献(注：内容为模拟概括，实际文献需通过学术数据库查询)：
1. **《HERC5 is an IFN-induced ISG15 E3 ligase that mediates ISGylation of viral targets》**
- 作者：Dzitko, K., et al.
- 摘要：研究揭示了HERC5作为干扰素刺激基因(ISG)编码的E3连接酶，通过重组蛋白实验证实其催化病毒蛋白的ISG化修饰，抑制病毒复制。
2. **《Recombinant HERC5 ubiquitin ligase exhibits potent antiviral activity in vitro》**
- 作者：Zhang, Y., et al.
- 摘要：通过大肠杆菌表达系统获得重组HERC5蛋白，证实其通过泛素化途径降解宿主限制因子，增强细胞抗RNA病毒能力。
3. **《Structural and functional analysis of the HERC5 C-terminal domain》**
- 作者：Li, T., & Wei, W.
- 摘要：利用重组HERC5蛋白的C端结构域进行晶体学分析，揭示其与泛素结合的关键位点，为靶向HERC5的药物设计提供依据。
如需具体文献，建议在PubMed或Web of Science中检索关键词“HERC5 recombinant protein”。
HERC5 (HECT and RCC1 domain-containing protein 5) is a member of the HERC family of ubiquitin E3 ligases, which play critical roles in protein post-translational modification and cellular regulation. Structurally, it contains an N-terminal RCC1-like domain (RLD) involved in guanine nucleotide exchange and a C-terminal HECT domain responsible for ubiquitin ligase activity. HERC5 is unique among HERC proteins due to its dual functionality: it not only catalyzes the covalent attachment of ubiquitin or ubiquitin-like proteins to target substrates but also acts as a primary enzyme for ISG15 (interferon-stimulated gene 15) conjugation, a process termed ISGylation.
This protein is highly induced by type I interferons (IFNs) and is integral to innate antiviral immunity. Studies show HERC5 restricts viral replication by ISGylating viral and host proteins, disrupting viral assembly or promoting their degradation. For example, it inhibits HIV-1 by targeting viral capsid proteins and modulates the activity of host factors like IRF3 to enhance antiviral signaling. Additionally, HERC5 interacts with the JAK-STAT pathway, linking ISGylation to broader immune responses.
Recombinant HERC5 protein, produced via heterologous expression systems (e.g., Escherichia coli or mammalian cells), enables mechanistic studies of its enzymatic activity and interactions. Its purification often involves affinity tags (e.g., His-tag) and optimization to maintain functional conformation. Research applications include exploring its role in viral pathogenesis, cancer (where dysregulation is observed), and autoimmune diseases. Despite progress, questions remain about its substrate specificity, regulatory mechanisms, and therapeutic potential as a target for immunomodulation or antiviral drug development.
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