| 纯度 | >90%SDS-PAGE. |
| 种属 | Arabidopsis |
| 靶点 | CAT3 |
| Uniprot No | Q42547 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-492aa |
| 氨基酸序列 | MDPYKYRPSSAYNAPFYTTNGGAPVSNNISSLTIGERGPVLLEDYHLIEKVANFTRERIPERVVHARGISAKGFFEVTHDISNLTCADFLRAPGVQTPVIVRFSTVVHERASPETMRDIRGFAVKFYTREGNFDLVGNNTPVFFIRDGIQFPDVVHALKPNPKTNIQEYWRILDYMSHLPESLLTWCWMFDDVGIPQDYRHMEGFGVHTYTLIAKSGKVLFVKFHWKPTCGIKNLTDEEAKVVGGANHSHATKDLHDAIASGNYPEWKLFIQTMDPADEDKFDFDPLDVTKIWPEDILPLQPVGRLVLNRTIDNFFNETEQLAFNPGLVVPGIYYSDDKLLQCRIFAYGDTQRHRLGPNYLQLPVNAPKCAHHNNHHEGFMNFMHRDEEINYYPSKFDPVRCAEKVPTPTNSYTGIRTKCVIKKENNFKQAGDRYRSWAPDRQDRFVKRWVEILSEPRLTHEIRGIWISYWSQADRSLGQKLASRLNVRPSI |
| 预测分子量 | 64.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CAT3重组蛋白的参考文献示例，基于相关领域常见研究主题的合理推测：
1. **文献名称**: "Heterologous Expression and Functional Analysis of Recombinant CAT3 in Arabidopsis thaliana"
**作者**: Thompson R, et al.
**摘要**: 研究报道了在拟南芥中异源表达CAT3重组蛋白，证实其过氧化氢酶活性显著增强，提高了植物对氧化胁迫的耐受性，为作物抗逆性改良提供依据。
2. **文献名称**: "Optimization of CAT3 Recombinant Protein Production in Pichia pastoris for Biomedical Applications"
**作者**: Chen L, et al.
**摘要**: 通过优化毕赤酵母表达系统的培养条件，实现CAT3重组蛋白的高效分泌表达，并验证其在体外清除自由基的能力，提示其在抗氧化治疗中的潜力。
3. **文献名称**: "Crystal Structure of Human CAT3 Recombinant Protein: Insights into Substrate Specificity"
**作者**: Müller S, et al.
**摘要**: 解析了人源CAT3重组蛋白的晶体结构，发现其底物结合口袋的独特构象，解释了其对特定代谢产物的催化偏好性，为设计靶向抑制剂奠定基础。
4. **文献名称**: "Recombinant CAT3 as a Therapeutic Agent in Murine Models of Neurodegeneration"
**作者**: Gupta A, et al.
**摘要**: 在小鼠神经退行性疾病模型中，重组CAT3蛋白通过血脑屏障有效减轻神经元氧化损伤，显著改善行为学指标，支持其作为神经保护剂的开发价值。
**备注**：以上文献为示例性内容，实际研究中请通过学术数据库(如PubMed、Web of Science)检索具体文献。若需进一步协助定位真实文献，请提供更多研究背景或关键词。
**Background of CAT3 Recombinant Protein**
CAT3 (Cationic Amino Acid Transporter 3), also known as SLC7A3. is a protein encoded by the *SLC7A3* gene in humans. It belongs to the solute carrier family 7 (SLC7), which comprises transporters critical for cellular uptake of cationic amino acids, such as arginine, lysine, and ornithine. These amino acids play vital roles in numerous biological processes, including nitric oxide synthesis, polyamine production, and protein metabolism. CAT3 is primarily expressed in specific tissues, such as the brain, liver, and immune cells, where it regulates nutrient supply and metabolic homeostasis.
Recombinant CAT3 protein is engineered using biotechnological methods, often through heterologous expression systems like *E. coli*, yeast, or mammalian cell cultures. The recombinant form allows researchers to study CAT3’s structure, transport mechanisms, and interactions in controlled settings. Its production typically involves cloning the *SLC7A3* gene into expression vectors, followed by purification via affinity chromatography.
Research on recombinant CAT3 has provided insights into its role in diseases. For example, dysregulation of cationic amino acid transport is linked to pathologies such as hypertension, neurodegenerative disorders, and cancer. CAT3’s involvement in arginine uptake also ties it to immune responses, as arginine metabolism influences T-cell function and macrophage activity. Additionally, recombinant CAT3 serves as a tool for drug discovery, enabling high-throughput screening of compounds targeting amino acid transporters.
Despite progress, challenges remain in understanding CAT3’s tissue-specific regulation and post-translational modifications. Ongoing studies aim to elucidate its physiological significance and therapeutic potential, particularly in conditions involving metabolic imbalance or immune dysfunction. The development of CAT3 recombinant protein thus represents a critical step in advancing both basic science and clinical applications.
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