| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ESYT2 |
| Uniprot No | A0FGR8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 191-662aa |
| 氨基酸序列 | DTERAEWLNKTVKHMWPFICQFIEKLFRETIEPAVRGANTHLSTFSFTKVDVGQQPLRINGVKVYTENVDKRQIILDLQISFVGNCEIDLEIKRYFCRAGVKSIQIHGTMRVILEPLIGDMPLVGALSIFFLRKPLLEINWTGLTNLLDVPGLNGLSDTIILDIISNYLVLPNRITVPLVSEVQIAQLRFPVPKGVLRIHFIEAQDLQGKDTYLKGLVKGKSDPYGIIRVGNQIFQSRVIKENLSPKWNEVYEALVYEHPGQELEIELFDEDPDKDDFLGSLMIDLIEVEKERLLDEWFTLDEVPKGKLHLRLEWLTLMPNASNLDKVLTDIKADKDQANDGLSSALLILYLDSARNLPSGKKISSNPNPVVQMSVGHKAQESKIRYKTNEPVWEENFTFFIHNPKRQDLEVEVRDEQHQCSLGNLKVPLSQLLTSEDMTVSQRFQLSNSGPNSTIKMKIALRVLHLEKRER |
| 预测分子量 | 61.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ESYT2重组蛋白的虚构参考文献示例(仅供格式参考，非真实文献)：
1. **文献名称**：Structural characterization of ESYT2 recombinant protein and its lipid transfer activity
**作者**：Smith A, et al.
**摘要**：研究通过X射线晶体学解析了ESYT2重组蛋白的C端结构域，揭示了其C2结构域与磷脂结合的分子机制，证实ESYT2在内质网-质膜接触位点的脂质运输中起关键作用。
2. **文献名称**：ESYT2-mediated membrane tethering regulates autophagy under nutrient stress
**作者**：Chen L, et al.
**摘要**：利用重组ESYT2蛋白进行体外膜结合实验，发现其通过VAP蛋白与内质网互作，动态调控自噬小体形成，为代谢应激下细胞膜重塑机制提供新证据。
3. **文献名称**：High-throughput screening identifies ESYT2 as a potential biomarker in triple-negative breast cancer
**作者**：Wang Y, et al.
**摘要**：通过重组ESYT2蛋白制备抗体，临床样本分析显示ESYT2在乳腺癌组织异常高表达，与患者预后负相关，提示其可作为治疗靶点。
4. **文献名称**：Recombinant ESYT2 facilitates ER-PM contact formation in yeast complementation assay
**作者**：Tanaka K, et al.
**摘要**：在酵母模型中表达人源重组ESYT2蛋白，成功挽救内质网-质膜接触缺陷表型，证实其功能在进化中的保守性，为膜接触研究提供新工具。
注：以上内容为模拟学术文献格式的虚构案例，实际研究中请通过PubMed/Google Scholar等平台检索真实文献。
ESYT2 (Extended Synaptotagmin-2) is a member of the evolutionarily conserved Extended Synaptotagmin (ESYT) protein family, which plays critical roles in membrane contact site (MCS) formation and lipid transfer between intracellular organelles. Structurally, ESYT2 contains an N-terminal transmembrane domain that anchors it to the endoplasmic reticulum (ER), followed by multiple C2 domains that mediate calcium-dependent interactions with plasma membrane phospholipids. This architecture enables ESYT2 to tether the ER to the plasma membrane, facilitating non-vesicular lipid exchange and regulating cellular processes such as lipid homeostasis, calcium signaling, and membrane repair.
The protein has garnered significant attention due to its involvement in various physiological and pathological conditions. Studies link ESYT2 to cancer progression, where its overexpression correlates with tumor metastasis and poor prognosis, potentially through modulation of lipid metabolism and signaling pathways. Additionally, ESYT2 interacts with neurodegenerative disease-related proteins, suggesting a role in neuronal membrane maintenance. Its function in insulin secretion and glucose metabolism further implicates it in metabolic disorders.
Recombinant ESYT2 proteins are typically produced using heterologous expression systems (e.g., HEK293 or insect cells) to study structure-function relationships, protein-protein interactions, and lipid transfer mechanisms in vitro. Tagged versions (e.g., His-tag, GST-tag) enable purification via affinity chromatography and application in biochemical assays. Researchers utilize these recombinant tools to dissect ESYT2's role in organelle communication, screen potential therapeutic compounds targeting ESYT2-associated pathways, and develop disease models. The development of active recombinant ESYT2 has significantly advanced our understanding of inter-organelle lipid dynamics and its implications in human health.
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