| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HY5 |
| Uniprot No | O24646 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-168aa |
| 氨基酸序列 | MQEQATSSLAASSLPSSSERSSSSAPHLEIKEGIESDEEIRRVPEFGGEAVGKETSGRESGSATGQERTQATVGESQRKRGRTPAEKENKRLKRLLRNRVSAQQARERKKAYLSELENRVKDLENKNSELEERLSTLQNENQMLRHILKNTTGNKRGGGGGSNADASL |
| 预测分子量 | 25.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HY5重组蛋白的3-4篇参考文献的简要概括(基于公开研究领域归纳，非真实文献)：
1. **《HY5 transcription factor mediates light-dependent regulation of plant development》**
- 作者：Oyama, T. 等(1997)
- 摘要：研究揭示了拟南芥HY5蛋白在光信号传导中的核心作用，通过重组HY5蛋白实验证实其直接结合下游靶基因启动子，调控光形态建成相关基因表达。
2. **《Recombinant HY5 protein purification and its DNA-binding characterization in Arabidopsis》**
- 作者：Lee, J. 等(2007)
- 摘要：报道了HY5重组蛋白在大肠杆菌中的高效表达与纯化方法，并通过凝胶迁移实验(EMSA)证明HY5特异性结合G-box顺式元件，解析其DNA结合结构域功能。
3. **《HY5 interacts with phytochrome B to regulate secondary metabolism in plants》**
- 作者：Zhang, Y. 等(2011)
- 摘要：利用重组HY5蛋白进行体外互作实验，发现HY5与光敏色素phyB直接互作，协同调控类黄酮生物合成通路，揭示其在光调控代谢中的新功能。
4. **《HY5-dependent oxidative stress tolerance mediated by recombinant protein overexpression in crops》**
- 作者：Chen, L. 等(2019)
- 摘要：通过在水稻中过表达重组HY5蛋白，证明其增强植物抗氧化胁迫能力，并激活胁迫响应基因，为作物抗逆育种提供分子依据。
(注：以上为模拟摘要，实际文献需通过数据库如PubMed、Web of Science检索确认。)
HY5 (ELONGATED HYPOCOTYL 5) is a pivotal transcription factor in plants, first identified in *Arabidopsis thaliana*, that plays a central role in light signaling and photomorphogenesis. It acts as a positive regulator of light-responsive gene expression, integrating signals from photoreceptors (e.g., phytochromes and cryptochromes) to orchestrate plant development under varying light conditions. HY5 is a basic leucine zipper (bZIP) protein that binds to promoter regions of target genes, modulating processes such as chlorophyll biosynthesis, anthocyanin accumulation, root development, and stress responses. Its activity is tightly regulated by COP1 (CONSTITUTIVE PHOTOMORPHOGENIC 1), which promotes HY5 degradation in darkness, ensuring skotomorphogenesis.
Recombinant HY5 protein is produced via heterologous expression systems (e.g., *E. coli* or yeast) to study its molecular functions *in vitro*. This engineered protein retains DNA-binding specificity and transcriptional activation capacity, enabling biochemical assays like electrophoretic mobility shift assays (EMSAs) and chromatin immunoprecipitation (ChIP). Researchers utilize recombinant HY5 to dissect its interaction networks with downstream genes (e.g., *CHS*, *RBCS*) and upstream regulators (e.g., COP1. HY5-HOMOLOG [HYH]).
Studies on HY5 have expanded to crops, revealing conserved roles in light adaptation and stress resilience. Its recombinant form is instrumental in biotechnological applications, such as optimizing plant growth under controlled environments or enhancing nutrient uptake. Recent work also explores HY5's involvement in circadian rhythms and cross-talk with hormone signaling pathways (e.g., auxin, gibberellin). Despite progress, questions remain about tissue-specific regulatory mechanisms and evolutionary diversification of HY5-like proteins across plant species. Overall, HY5 recombinant protein serves as a critical tool for unraveling light-mediated developmental plasticity in plants. (Word count: 298)
×
